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Mouse Perforin 1 (PRF1) ELISA Kit-Sandwich

Cat. No.EK5F604

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Perforin 1 (PRF1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse PRF1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Perforin 1 (PRF1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Profilin-1 interacts with actin monomers and plays a crucial role in organizing the actin cytoskeleton. It prevents actin polymerization at high concentrations but promotes it at low levels. In mouse breast cancer cells, Profilin-1 has a dual function: it aids tumor growth by enhancing cell proliferation and survival, and it also modulates tumor suppressor activity through its nuclear localization and phosphorylation at Ser-137. This phosphorylation at Ser-137 disrupts Profilin-1's interaction with its PLP site, lifting the inhibition on cell cycle arrest and increasing sensitivity to apoptosis, thus offering a potential target for cancer treatment.

Target/Biomarker Mouse PRF1
Target Synonym FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
Gene ID 816495
UniProt ID Q42449

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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