DNA veterinary vaccines carry the DNA of infectious agents in animals, thereby expressing disease-related antigens. Unlike conventional vaccines, DNA vaccines do not contain live pathogens and cause disease but have the ability to induce a protective immune response from the immune system. Following the footsteps of advanced technology, BioVenic provides DNA veterinary vaccine development services to help you develop highly effective and safe veterinary vaccines to resist animal pathogens and maintain animal health.
Our Services
The process of DNA veterinary vaccine development is relatively simpler compared to traditional veterinary vaccine types. BioVenic provides full-process DNA veterinary vaccine development services and optimizes the process in multiple steps to meet your development needs.
Fig.1 DNA Vaccine Development Process.1
Plasmid Design and Construction
BioVenic provides molecular cloning services following the selection of target antigen genes of the pathogen. The process involves synthesizing the target gene sequence and then constructing it into an insert fragment, utilizing restriction enzymes. We offer the enzymes and buffers required for ligation reactions and continuously monitors the efficiency of the ligation process.
Plasmid Transformation
BioVenic offers plasmid transformation services for DNA veterinary vaccine development. Recombinant DNA is transformed into recipient cells (such as Escherichia coli) using microbial transformation techniques, allowing the target gene in the recombinant DNA to replicate or express in the recipient cells, thereby altering the original characteristics of the host cells or producing new substances.
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Plasmid Transformation Services
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Description
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Heat Shock
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After mixing plasmid DNA with host cells, a brief exposure to a high-temperature 'heat shock' (usually around 42°C) is applied, increasing the uptake of plasmid DNA by the cells.
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Electroporation
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In electroporation, an electrical pulse is applied to the cell-plasmid mixture, creating temporary pores in the cell membrane through which plasmid DNA can enter the cell.
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Plasmid Production and Purification
Our plasmid preparation and purification services in DNA veterinary vaccine development are able to efficiently generate and purify plasmids containing target genes for you to ensure high-quality DNA during vaccine preparation. During the cultivation of transformed bacteria, our established cultivation platform provides a high-quality growth medium and a contamination-free environment. This allows for the efficient replication of plasmids and further amplification of the copy number of the target gene. BioVenic also offers various purification methods to effectively purify plasmids from bacterial cultures, removing impurities and other biological molecules.
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Plasmid Purification Services
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Description
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Ethanol Precipitation
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BioVenic offers the cost-effective ethanol precipitation method, which can alter the DNA structure, causing plasmid DNA to precipitate from the solution. After removing soluble salts and organic small molecules, the plasmid DNA precipitate is then resuspended.
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Spin Column-Based Purification
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BioVenic provides solid-phase extraction services for the rapid purification of plasmid DNA. Through a four-step process involving lysis, binding, washing, and elution, plasmid DNA selectively binds to a silicon dioxide membrane. Unbound impurities are washed away, leaving the desired plasmid DNA eluted.
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Plasmid Screening and Characterization
In order to ascertain whether transformed colonies contain the target insert fragment, BioVenic offers plasmid clone screening services with multiple validation methods.
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Plasmid Screening and Characterization Services
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Description
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Antibiotic Resistance Screening
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BioVenic offers Antibiotic Resistance Screening services to ensure the stability and selectivity of the constructed expression vector in host cells. By introducing antibiotic resistance genes, we screen for successfully transformed cell populations, thereby ensuring the effective expression vector is maintained throughout the subsequent vaccine production process.
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Fluorescent Labeling
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BioVenic provides fluorescence microscopy or fluorescence identification equipment to screen for luminescent or fluorescent bacterial clones containing fluorescent markers or reporter genes.
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Blue and White Spot Screening
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We provide Blue and White Spot Screening services for detecting the success of DNA insertion. Using a culture medium with X-gal staining, bacteria with successfully inserted target DNA form blue spots, while those without insertion appear white.
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PCR Screening
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We help design specific primers so that only clones with the target sequence to produce PCR products for plasmid screening.
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The main reason for the lower immunogenicity of DNA veterinary vaccines is believed to be the inefficient expression of target antigen proteins in immune-related cells and tissues, leading to insufficient antigen load. The low protein expression level is primarily due to the poor uptake of DNA veterinary vaccine plasmids by antigen-presenting cells. To enhance absorption, the choice of delivery route for DNA vaccines becomes crucial. In your vaccine development process, Company guides on selecting the appropriate DNA veterinary vaccine delivery route and further optimizes it based on the results of subsequent immunogenicity assessments.
Fig.2 Physical DNA vaccine delivery technologies.3
Delivery by Patch
Transdermal immunization typically involves the initial step of gently abrading the stratum corneum before applying the vaccine. Subsequently, a sealing patch is applied to allow the vaccine to be absorbed into the skin.
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DNA Tattooing
This involves applying the DNA vaccine directly to the shaved skin surface and using a rotary tattoo machine to introduce the plasmid immunogen into the dermis.
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Gene Gun
With the assistance of high-pressure helium gas, spherical gold or tungsten particles coated with DNA are accelerated into host cells.
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Delivery by Microneedles
Using tiny needles or microneedle arrays, DNA or other biomolecules are directly delivered into the skin.
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Our company assists in the selection of the most suitable DNA vaccine delivery route and further optimization based on the results of immunogenicity assessments to facilitate improved absorption and immunogenicity.
Why Choose Us?
Plasmids delivered promptly, ensuring batch consistency.
Professional laboratory personnel to assist in efficient transformation.
Various delivery systems to meet animal vaccine development needs.
Due to their high safety and ease of production, DNA veterinary vaccines have already attracted the attention of many researchers. The DNA veterinary vaccine development services offered by BioVenic come with significant advantages. Leveraging advanced technological platforms, we achieve efficient and personalized DNA veterinary vaccine design, help you enhance the immune protection of animals against specific pathogens. Please contact us , and we will be dedicated to serving you wholeheartedly!
References
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Wang, Xingyun, et al. "Development of DNA vaccine candidate against SARS-CoV-2." Viruses 14.5 (2022): 1049.
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Porter, Kevin R., and Kanakatte Raviprakash. "DNA vaccine delivery and improved immunogenicity." Current issues in molecular biology 22.1 (2017): 129-138.
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Shafaati, Maryam, et al. "A brief review on DNA vaccines in the era of COVID-19." Future Virology 17.1 (2022): 49-66.
