Gene Delivery Solutions in Precision Animal Breeding

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The successful implementation of CRISPR technology relies on the acquisition, efficient delivery, and safe transfer of the technology to the desired cell or tissue in order to achieve its editing effect. BioVenic specializes in the development and enhancement of editing machine delivery methods. We offer customized services to precision animal breeding researchers worldwide, providing effective delivery solutions for various virus-mediated and physical and chemical-related applications. We assist in improvement and optimization of key aspects of animal gene delivery system, such as loading capacity, immunogenicity, and gene delivery effectiveness, to ensure that customers receive high-quality and personalized services.

Fig.1 Schematic Diagram of CRISPR Editing Machine Delivery Methods.¹

Gene Delivery Services in Precision Animal Breeding

Virus-mediated Gene Delivery

Virus-mediated gene delivery has been widely applied in animal genome editing research. BioVenic offers personalized services for the three most prevalent viral vectors, which include adenoviral vectors, adeno-associated virus vectors, and lentiviral vectors. We match suitable carrier type and technical process for specific research project. We offer a series of services, including design, packaging, and concentration purification, and deliver satisfactory results with high titer and quality.

Liposome-mediated Gene Delivery

The CRISPR system can be delivered in the form of plasmid vectors, gRNA), or protein lipid-based nanoparticles (LNPs) designed by changing the internal charge and additional lipid components for genome editing in animals. LNPs can be targeted to specific tissues through molecular design. BioVenic's custom-designed liposome-mediated gene delivery service seamlessly aligns with the requirements of your scientific research project.

Electroporation

Electroporation is a physical delivery method that applies a pulse of electrical current to stimulate pores in the cell membrane, momentarily opening them and allowing DNA to enter the cell. The advantage of electroporation technology is that it is suitable for in vitro and in vivo gene editing of almost all types of cells. Through extensive optimization of the delivery ratio of specific cell types and the perforation process, BioVenic offers customers high-efficiency, low-toxicity, and no loss of editing efficiency electroporation research customization services.

DNA Microinjection

Microinjection can deliver all types of CRISPR cargo into target cells, and the utilization of microneedles under a microscope can achieve precise gene delivery to any specific location within the cell. Microinjection is not limited by the size of the CRISPR cargo and is highly efficient, without affecting surrounding tissues or cells. BioVenic has a professional technical team skilled in operating microinjection technology, which ensures that the entire process is accurate and efficient, enabling you to easily complete this challenging experimental operation and advance scientific research projects.

Comparison of Gene Delivery Solutions for Precision Animal Breeding

Solutions	Viral Delivery			Non-Viral Delivery
Solutions	Adenovirus (AV)	Adeno-associated virus (AAV)	Lentivirus (LV)	Lipid-Based Nanoparticles (LNPs)	Microinjection	Electroporation
Delivery Format	DNA	DNA	DNA	DNA, mRNA or protein	DNA, mRNA or protein	DNA, mRNA or protein
Applications	In vivo	In vivo	In vitro and ex vivo	In vitro and in vivo	In vitro and ex vivo	In vitro and ex vivo
Packaging Capability	~ 8.3 kb	~ 4.7 kb	~ 6.4 kb	nM levels of Cas9 and sgRNA	nM levels of Cas9 and sgRNA	nM levels of Cas9 and sgRNA
Efficiency	＋＋	＋＋	＋＋＋	＋	＋＋＋＋	＋＋＋
Safety concern	＋＋＋	＋	＋＋	＋	＋	＋
Advantages	No integration to the genome. High-level transient expression.	Minimal immunogenicity Long-term expression in nondividing cells. Nonpathogenic.	Large packaging capacity. No pre-existing immunogenicity.	FDA-approved. Low stress to the cells. High biocompatibility. Low immunogenicity.	Highly specific. Reproducible. Dosage more controllable.	Efficient delivery. Easy to operate.
Limitations	Immune response.	Limited cloning and packaging capacity.	Random integration. Insertional mutagenesis.	Relatively low delivery efficiency. Variable efficiency.	Low throughput. Technical challenging.	Cell viability issue. Significant stress on cell.

CRISPR-based Genome Editing Services for Farmed Animals

BioVenic has mastered the cutting-edge CRISPR-based animal genome editing technology, while providing customized gene delivery solutions for the efficient and safe gene delivery of editing tools to cells or tissues. As a dependable partner on your scientific research journey, we provide one-stop CRISPR solutions for precise breeding to fulfill your diverse scientific research requirements. Click on the link below to learn more about these services.

Why Choose Us?

Diverse gene delivery solutions available for customization.

Design and optimization of the program for precision animal breeding.

One-stop CRISPR genome editing service customization for farmed animals: from target design to editing machine delivery to result verification.

Accurate, safe and efficient delivery.

R & D team with rich experience in practical operation.

BioVenic relies on its expert team to provide highly customized gene delivery design solutions. Our experts and advanced technology platforms ensure the smooth progress and high-quality completion of the project at both the academic and technical levels. If you are conducting research on genome editing in farmed animals and facing challenges with the accurate and efficient delivery of genes, please do not hesitate to contact us for assistance.

Reference

Demirci, Selami, et al. "Advances in CRISPR Delivery Methods: Perspectives and Challenges." The CRISPR Journal 5.5 (2022): 660-676.