This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
ELISA Assay Service
BioVenic provides ELISA-based detection services for various sample types-including animal serum, tissues, cells, urine, and feed-supporting veterinary research through the quantitative analysis of both macromolecular and small-molecule markers. Utilizing methods such as sandwich and competitive ELISA, BioVenic can perform assays using our existing kits or by developing custom kits tailored to your specific needs.
Introduction
BioVenic empowers the global scientific community with precision, high-sensitivity, and publication-ready data to accelerate animal health and veterinary medicine research. Built upon our comprehensive animal-focused R&D platforms, BioVenic's ELISA Assay Service handles everything from routine analysis to high-throughput screening. Whether you need custom target detection, large-scale sample validation or high quality commercial kits, our experts deliver reliable, robust data so that you can fully focus on your next scientific breakthrough.
Discuss Your Project With Our Experts
Service Overview
BioVenic provides professional ELISA testing services specifically designed for a wide range of animal species and complex biological matrices. With a strong menu of over 5,000+ validated targets, we specialize in accurate quantitative and qualitative analysis across multiple research scopes.
Broad Research Portfolio:
Specialized solutions for Oncology, Immunology & Inflammation, Neuroscience, Metabolism and Epigenetics.
Comprehensive Methodological Configurations:
- Sandwich ELISA: Ideal for high specificity quantification of macromolecules, proteins and cytokines.
- Competitive ELISA: Specialised for small molecules, hormones, lipids and short peptides.
- Indirect ELISA: Commonly used for qualitative or semi-quantitative screening of target antibodies in animal serum in the context of vaccine evaluation or infection profiling.
- Double-Antigen Sandwich ELISA: Designed for species-independent detection of specific animal immunoglobulins.
Sample Requirements
For maximum assay sensitivity and to provide sufficient volume for possible confirmation or dynamic dilution re-runs, we suggest the following sample specifications:
1. Accepted Sample Types & Recommended Volumes
Serum / Plasma: Requires 50-100 µL per well. We recommend submitting >200-500 µL per sample. Serum must be separated immediately via centrifugation; hemolysis must be strictly avoided as it releases endogenous peroxidases that cause high background or false positives. For plasma, please specify the anticoagulant used (EDTA or Heparin is preferred).
Cell Culture Supernatant: We recommend submitting >500-1,000 µL per sample. Centrifuge to remove cell debris prior to aliquoting and freezing. If the culture medium contains high concentrations of FBS, please inform us during inquiry to address potential cross-reactivity with bovine homologous proteins.
Tissue Homogenates: We recommend providing >50-100 mg of fresh tissue. Samples can be pre-homogenized in a lysis buffer containing protease inhibitors, or submitted directly as flash-frozen fresh tissues for processing by our lab team.
Other Samples: Urine, Fecal Extracts, Bronchoalveolar Lavage Fluid (BALF), Saliva, Cerebrospinal Fluid (CSF), Ascites, Semen, and Milk. We recommend providing >300-500 µL per sample. For special samples such as animal feed, please consult our technical staff before collecting and shipping them.
Biosafety Notice: To maintain strict laboratory biosafety compliance, BioVenic does not accept un-inactivated samples containing high-consequence, zoonotic pathogens. All high-risk samples must be accompanied by inactivation certification or undergo safety review by our technical team prior to shipment.
2. Sample Submission
Please consult our technical support team in advance to confirm that your sample type is suitable for the required testing, and to confirm the required sample volume for specific sample types or parameters before shipping your samples. Then, follow our instructions to collect, label, and package the samples before shipping them to our address.
Service Workflow
We execute a highly structured, fully traceable, and guideline-driven workflow to ensure maximum data integrity at every milestone:
Fig 1. BioVenic ELISA Assay Service Workflow
Unsure about your sample compatibility or required dilution factors? Let our technical experts review your experimental parameters before you ship your samples.
Multi-Species Versatility & Customized Solutions
Unlike conventional platforms focused exclusively on human or standard rodent targets, BioVenic excels in breaking species barriers. We support livestock, aquaculture, companion animals, and other animal research via three flexible routes:
- Full-Service In-House Assays: Direct sample testing using our verified antibody pairs.
- Commercial Kit Procurement: Order from our extensive line of Animal Immunoassay Kits to perform independent testing in your own facility.
- De Novo Customization: For rare or novel biomarkers absent from commercial catalogs, we provide a complete Veterinary ELISA Development pipeline spanning antigen design, antibody pair generation, and assay validation.
| Classification | Common Biomarker Targets Covered | Solutions & Product Links |
|---|---|---|
| Canine / Feline |
|
Canine ELISA Kits Feline ELISA Kits |
| Bovine / Porcine / Ovine |
|
Bovine ELISA Kits Swine ELISA Kits Sheep and Goat ELISA Kits |
| Poultry / Avian |
|
Avian ELISA Kits |
| Equine |
|
Equine ELISA Kits |
| Aquaculture Species |
|
Aquaculture Species ELISA Kits |
| Model Organisms |
|
Rodent ELISA Kits |
| Food & Feed |
|
Food and Feed Safety Assay Kits |
Case Study
Rigorous, compliant, and traceable data packages form the bedrock of BioVenic's services. Every technical report we deliver contains raw instrument readings alongside mathematically verified curve fittings.
1. Project Background
A research team partnered with BioVenic to quantify a high-molecular-weight protein biomarker within animal serum samples. Based on pre-project consultations, a highly sensitive in-house Sandwich ELISA was selected, and replication parameters (singlicate, duplicate, or triplicate) were aligned with the client.
To guarantee uncompromised assay performance, a strict analytical pipeline was enforced:
- Quality Assurance: The kit's analytical performance was rigorously validated, with a formal COA.
- Pilot Optimization: pilot runs were conducted to pinpoint the absolute optimal dilution factors.
- Traceable Processing: Upon receipt, specimens were assigned unique internal labels for testing and processed according to standardized kit manuals.
- Anomalous Value Flagging: Statistical outliers or atypical biological readings were flagged for subsequent client technical feedback.
2. Standard Curve Generation
To account for saturation effects, we utilize advanced biostatistical software to compute a Logistic Regression.
Fig 2. Representative ELISA Standard Curve with high-precision regression fitting (R^2 = 0.9949).
3. Data Reporting
Our data packages provide absolute transparency, enabling seamless back-calculation from raw absorbance numbers to final adjusted concentration values under multiple dilution factors.
Table. 1 OD450 raw data (sample, partial)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | 2.262 | 2.1798 | 1.2117 | 1.2181 | 0.7154 | 0.7085 | 0.3513 | 0.334 | 0.1549 | 0.1482 | 0.1225 | 0.1026 |
| B | 0.174 | 0.1945 | 0.2646 | 0.1333 | 0.1937 | 0.3602 | 0.2954 | 0.2469 | 0.1772 | 0.3026 | 0.3528 | 0.2628 |
| C | 0.3722 | 0.3807 | 0.4125 | 0.3125 | 0.2591 | 0.208 | 0.3919 | 0.4081 | 0.2664 | 0.2378 | 0.3682 | 0.2478 |
| D | 0.2513 | 0.4061 | 0.4014 | 0.3164 | 0.2233 | 0.399 | 0.4696 | 0.4849 | 0.3398 | 0.3689 | 0.2965 | 0.2225 |
Table. 2 Calculated result (sample, partial)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | 19.9890 | 19.2430 | 10.4560 | 10.5141 | 5.9513 | 5.8887 | 2.6466 | 2.4896 | 0.8639 | 0.8031 | 0.5699 | 0.3892 |
| B | 1.0373 | 1.2234 | 1.8596 | 0.6679 | 1.2161 | 2.7274 | 2.1392 | 1.6990 | 1.0664 | 2.2045 | 2.6602 | 1.8433 |
| C | 2.8363 | 2.9134 | 3.2021 | 2.2944 | 1.8097 | 1.3459 | 3.0151 | 3.1621 | 1.8760 | 1.6164 | 2.8000 | 1.7072 |
| D | 1.7389 | 3.1440 | 3.1013 | 2.3298 | 1.4848 | 3.0795 | 3.7203 | 3.8592 | 2.5422 | 2.8063 | 2.1492 | 1.4775 |
Deliverables
Upon project completion, BioVenic compiles an exhaustive technical dossier containing:
- Raw Microplate Reader Readouts: Unaltered digital raw files exported directly from the spectrophotometer.
- Comprehensive Sample Map & Plate Layout: Transparent mapping of client IDs, re-numbered internal IDs, standards, and blanks.
- Dual Standard Curves: Full regression parameters for both standard curves generated during the assay setup.
- Calculated Analyte Concentrations: Final target concentrations for all unknown samples, pre-adjusted for linear dilution factors.
- Outlier & Anomaly Reports: Detailed call-outs and annotations for any samples demonstrating anomalous readings.
- Certificate of Analysis (COA): Quality control documentation validating kit performance and batch integrity.
- Complete ELISA Service Report: A formal dossier detailing experimental materials, standardized methodologies, instrumentation profiles, and a concise synthesis of results.
| COA Sample | Kit Manual Sample (partial) |
|
|
Why Choose BioVenic?
5,000+ Validated Targets
Skip months of expensive, high-risk antibody pairing. Our comprehensive library offers off-the-shelf readiness for specialized veterinary markers.
Unrivaled Matrix Expertise
Advanced sample processing techniques allow us to mitigate interference from complex tissue homogenates and lipid-rich sera seamlessly.
Transparent & Complete Deliverables
We do not just send a final spreadsheet of numbers. Your data case file includes the original raw OD matrix, mathematical curve fitting equations, instrument logs, batch traceability, and a comprehensive COA.
Strict Confidentiality
Your intellectual property is protected by strict internal data firewalls. We adhere to a rigid non-disclosure policy; your findings will never be leaked or used for promotional case studies without explicit, written consent.
FAQs
Q1: How do you handle samples with unknown biomarker concentrations?
A1: We mitigate risk through small-scale pilot runs. For unknown samples, our team processes a representative subset across a series of linear dilution factors (e.g., 1:2, 1:5, 1:10, 1:50) against internal benchmarks. This pinpoints the ideal dilution factor required to ensure your final sample cohort fits safely within the most sensitive dynamic range of the standard curve.
Q2: What is the absolute minimum sample volume required for a duplicate run?
A2: While standard wells require 50-100 µL (amounting to 100-200 µL for duplicates), we highly recommend providing 200-500 µL per sample. This buffer protects against instrument dead volumes and secures adequate volume if confirmatory re-runs are necessary. If you are handling rare micro-volume samples (e.g., murine tear fluid or CSF), contact us ahead of time to arrange specialized micro-volume protocols.
Q3: Can you help if my target biomarker is not listed in your standard catalog?
A3: Absolutely. Through our dedicated Veterinary ELISA Development Service, our scientists can design custom antigens, generate high-affinity monoclonal antibody pairs, and configure a customized ELISA system for entirely novel or highly niche animal targets.
Q4: What are the sample shipping requirements?
A4: To prevent protein degradation, samples should be aliquoted into standard cryovials and shipped via air freight with ample dry ice.
Q5: What are your criteria for data quality control, specifically regarding CV (Coefficient of Variation) values?
A5: BioVenic enforces industry-leading bioanalytical standards for every microplate run. For standard curve validation and sample replicates, we strictly monitor two metrics: Intra-assay CV (Precision within the same plate): Maintained at < 8% for standard points and typically < 10% for sample replicates. Inter-assay CV (Consistency between different plates/runs): Maintained at < 12%.
Q6: In what format will the final data be delivered? Can you provide raw files for publication?
A6: We deliver a comprehensive, publication-ready Excel/PDF data package that ensures full transparency and traceability. The deliverables include:
- Raw OD450 Absorbance Matrix: The exact, unaltered digital readouts extracted directly from our microplate readers.
- Standard Curve Equations: The exact mathematical parameters derived from regression models.
- Concentration Calculation Sheets: Fully back-calculated concentrations for all unknown samples, pre-adjusted for your specific dilution factors.
- Certificate of Analysis (COA): Formal documentation signing off on the plate's QC metrics, instrumentation profiles, and operator logs.
Q7: Do you offer data analysis or statistical interpretation services (e.g., T-tests, ANOVA) for group comparisons?
A7: Our standard ELISA service includes comprehensive data reduction (transforming raw OD values into absolute concentration data). If your study design requires advanced biostatistical graphing or group-to-group comparative analysis (such as Student's t-tests, one-way/two-way ANOVA, or biomarker correlation curves) for publication or filing purposes, please indicate this during your initial inquiry. Our biostatistics team can append a customized bio-informatics analysis module to your project scope.
Published Data Demonstrates the Advantages of the ELISA Method
In the landmark study "Comparison of three different methods for the quantification of equine insulin," researchers addressed a critical veterinary challenge: the significant discrepancies in measuring horse insulin levels due to the misuse of human-specific diagnostic tools. The central focus of the literature was to evaluate the clinical accuracy of different immunoassays for diagnosing equine insulin dysregulation and metabolic syndrome. Within this comparative research, the ELISA emerged as a top-tier, highly reliable methodology. The ELISA demonstrated outstanding analytical performance, boasting an exceptional 98% ± 4% recovery rate upon dilution and superior linearity when measuring both basal and highly elevated insulin levels from dynamic stimulation tests.
Fig .3. Recovery upon dilution (RUD) of equine insulin in the three assays investigated: an ELISA (98 ± 4 %), b RIA (160 ± 41 %) and c.1,
Ready to Accelerate Your Research?
Entrust your immunoassay workflows to BioVenic's specialists. Skip the trial-and-error and fast-track your project with professional testing. Contact our technical support team today for a free project assessment and a custom quotation!
Reference
- Warnken, T et al. "Comparison of three different methods for the quantification of equine insulin." BMC veterinary research vol. 12,1 196. 9 Sep. 2016, https://doi.org/10.1186/s12917-016-0828-z. Distributed under Open Access license CC BY 4.0. Without modification.