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Mouse Glyoxalase I (GLO1) ELISA Kit-Sandwich

Cat. No.EK5F152

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Glyoxalase I (GLO1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse GLO1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Glyoxalase I (GLO1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 78.25-5000 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Lactoylglutathione lyase, also known as Glo1, catalyzes the conversion of hemimercaptal, produced from methylglyoxal and glutathione, into S-lactoylglutathione. It plays a role in regulating TNF-induced transcriptional activity of NF-kappa-B and is essential for normal osteoclastogenesis. In mice, GLO1 serves various biological functions, including anti-oxidation, anti-glycosylation, metabolic regulation, tumor suppression, and neuroprotection. GLO1 is often overexpressed in many cancers and is associated with tumor progression. In a mouse fibrosarcoma model, GLO1 overexpression and its nuclear translocation are closely linked to tumor progression.

Target/Biomarker Mouse GLO1
Target Synonym GLY-I; GLOD1; Glyoxalase Domain Containing 1; Lactoylglutathione lyase; Aldoketomutase; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase
Gene ID 109801
UniProt ID Q9CPU0

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Bibars, Roaa S, and Qosay A Al-Balas. "Computational fragment-based drug design of potential Glo-I inhibitors." Journal of enzyme inhibition and medicinal chemistry vol. 39,1 (2024): 2301758.
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