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Mouse Alpha-Endorphin (aEP) ELISA Kit-Competitive

Cat. No.EK5F766

Product TypeAnimal Immunoassay Kits

Size 96T

Product Overview

BioVenic Mouse Alpha-Endorphin (aEP) ELISA Kit-Competitive is designed for the quantitative determination of Mouse aEP in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Competitive ELISA method. For research use only.

Specifications

Assay Type ELISA-Competitive
Specificity The assay kit is specific for Mouse Alpha-Endorphin (aEP).
Target Species Mouse
Species Reactivity Mouse
Detection Range 15.7-1000 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

α-Endorphin demonstrates a significant inhibitory effect in the mouse spleen by suppressing antibody production. This effect is mediated through its binding to opiate-like receptors on splenocytes, and it can be blocked by naloxone, suggesting its mechanism is linked to opioid receptors. Compared to β-endorphin, α-endorphin is more potent in inhibiting antibody production. While β-endorphin can enhance the lymphoproliferative response of mouse splenocytes, α-endorphin does not exhibit this effect, highlighting differences in their immune regulation mechanisms. As a precursor of opioid peptides, α-endorphin may be expressed in various tumors, implying it could have a role in tumor biology.

Target/Biomarker Mouse aEP
Target Synonym AEP

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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