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Recombinant Mouse Glutamate-Cysteine Ligase Catalytic Subunit (Gclc), N-His

Cat. No.AP4C970

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Mouse Glutamate-Cysteine Ligase Catalytic Subunit (Gclc), N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 24.2 kDa. The purity is> 97%.

Specifications

Type Recombinant Protein
Species Mouse
Expression System E.coli
Purity > 97%
Endotoxin <1.0 EU per 1µg (determined by the LAL method)
Predicted Molecular Weight 24.2 kDa
Physical State Lyophilized
Formulation 20 mM Tris, 150 mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Target Information

Mouse Glutamate-Cysteine Ligase Catalytic Subunit (Gclc) is a key enzyme in the synthesis of glutathione, a major antioxidant in cells. It catalyzes the first and rate-limiting step in glutathione production by forming the γ-glutamyl-cysteine intermediate. Gclc is essential for maintaining cellular redox balance and protecting cells from oxidative stress. In mouse models, Gclc deficiency leads to increased oxidative damage and sensitivity to pro-oxidant agents. Additionally, Gclc plays a role in detoxification processes and is involved in the regulation of immune responses.

Protein Mouse Glutamate-Cysteine Ligase Catalytic Subunit (Gclc)
Protein Synonym GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase
Gene ID 14629
EC No. P97494

Shipping and Storage

This product is shipped with ice packs. Lyophilized protein can be stored at -80°C for 1 year. After reconstitution, the protein solution can be stored at 2-8°C for 2-7 days.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in deionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimize freeze-thaw cycles.
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