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Mouse Protamine 2 (PRM2) ELISA Kit-Sandwich

Cat. No.EK6F550

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Protamine 2 (PRM2) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse PRM2 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Protamine 2 (PRM2).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Protamine-2 (Prm2) in mice is a specialized protamine that plays a critical role in sperm maturation. It is essential for several processes, including chromatin compaction, DNA protection, antioxidative stress defense, and maintaining sperm morphology and function. The absence of Prm2 diminishes the antioxidant capacity of sperm, leading to the downregulation of key antioxidant enzymes such as SOD1 and PRDX5, and triggering a cascade of oxidative stress-related damage. Protamine-2 is a small, arginine-rich protein that binds to DNA, compacting sperm chromatin to protect it from external damage, including DNA degradation and oxidative stress.

Target/Biomarker Mouse PRM2
Target Synonym CT94.2; Cancer/Testis Antigen Family 94;Member 2; Sperm histone P2; Sperm protamine P2
Gene ID 19119
UniProt ID P07978

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Schneider, Simon et al. "Protamine-2 Deficiency Initiates a Reactive Oxygen Species (ROS)-Mediated Destruction Cascade during Epididymal Sperm Maturation in Mice." Cells/ vol. 9,8 1789. 27 Jul. 2020.
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