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Mouse Biotinidase (BTD) ELISA Kit-Sandwich

Cat. No.EK6F768

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Biotinidase (BTD) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse BTD in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Biotinidase (BTD).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Biotinidase catalytically releases biotin from biocytin, a product of the degradation of biotin-dependent carboxylases. As a hydrolase, biotinidase cleaves biotin from biotinylated peptides (such as biotin-ε-lysine) and from biotin itself (e.g., biotin resulting from degradation by biotinylase), thus releasing free biotin for cellular reuse. This process is crucial for maintaining the biotin cycle, as biotin serves as a coenzyme for numerous carboxylases involved in vital metabolic processes like glycolysis, fatty acid synthesis, and amino acid metabolism. Biotinidases are particularly important in the nervous system, playing significant roles in neuronal myelination and axon regeneration.

Target/Biomarker Mouse BTD
Target Synonym Biotinase
Gene ID 26363
UniProt ID Q8CIF4

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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