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Mouse O-GlcNAcase (OGA) ELISA Kit-Sandwich

Cat. No.EK5F106

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse O-GlcNAcase (OGA) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse OGA in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse O-GlcNAcase (OGA).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.32-20 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Protein O-GlcNAcase specifically removes GlcNAc, but not GalNAc, from O-glycosylated proteins. It deglycosylates a wide range of proteins, including CRYAB, ELK1, GSDMD, LMNB1, and TAB1. The enzyme can utilize p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates, but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). In mice, O-GlcNAcase is encoded by the Oga gene. O-GlcNAcase plays an important role in metabolic homeostasis in mice, especially during pregnancy and development. Studies have shown that mice with conditional knockout of O-GlcNAcase develop metabolic disorders and semi-lethal placental premature aging.

Target/Biomarker Mouse OGA
Target Synonym MEA5; MGEA5; NCOAT
Gene ID 76055
UniProt ID Q9EQQ9

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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