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Mouse Interleukin 35 (IL35) ELISA Kit-Sandwich

Cat. No.EK5F926

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Interleukin 35 (IL35) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse IL35 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Interleukin 35 (IL35).
Target Species Mouse
Species Reactivity Mouse
Detection Range 7.82-500 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

IL-35 is a heterodimeric cytokine that belongs to the IL-12 family and demonstrates significant immunosuppressive and regulatory effects in mice. It consists of two subunits: the IL-12α chain (p35) and EBI3. While IL-35 shares structural similarities with other IL-12 family members, it possesses distinct immunosuppressive properties. Primarily secreted by regulatory T cells (Tregs), IL-35 can also be produced by CD8+ T cells, activated dendritic cells (DCs), and regulatory B cells (Bregs). It exerts its immunosuppressive effects by inhibiting Th1 cell differentiation while promoting the formation of Th2 and Tregs. Furthermore, IL-35 helps maintain immune tolerance by suppressing the activation of effector T cells and macrophages.

Target/Biomarker Mouse IL35
Target Synonym IL-35; CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Lian, Min et al. "Interleukin-35 Regulates Immune Microenvironment of Autoimmune Hepatitis Through Inducing the Expansion of Myeloid-Derived Suppressor Cells." Frontiers in immunology vol. 10 2577. 7 Nov. 2019.
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