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Mouse Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit-Sandwich

Cat. No.EK6F535

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse HADHb in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb).
Target Species Mouse
Species Reactivity Mouse
Detection Range 1.57-100 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

In mice, β-hydroxyacyl-CoA dehydrogenase (β-HAD) plays a critical role in the β-oxidation of fatty acids. As a key enzyme, β-HAD catalyzes the β-oxidation of short-chain and medium-chain fatty acids, thereby providing energy for cellular processes. β-HAD is expressed in the liver, heart, and other tissues and is integral to fatty acid β-oxidation. Research indicates that β-HAD activity is influenced by high-fat diets or obesity; for example, in the hearts of obese mice, β-HAD activity is reduced, potentially leading to decreased fatty acid oxidation. Additionally, dietary interventions can affect β-HAD activity. An olive oil diet, for instance, can enhance β-HAD activity in the myocardium, thereby alleviating oxidative stress.

Target/Biomarker Mouse HADHb
Target Synonym MTPB; Mitochondrial Trifunctional Protein;Beta Subunit; 3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit; Beta-ketothiolase

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Castejón, María Luisa et al. "Potential Protective Role Exerted by Secoiridoids from Olea europaea L. in Cancer, Cardiovascular, Neurodegenerative, Aging-Related, and Immunoinflammatory Diseases." Antioxidants (Basel, Switzerland) vol. 9,2 149. 10 Feb. 2020.
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