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Recombinant Mouse Group IID Secretory Phospholipase A2 (Pla2g2d), N-His

Cat. No.AP4C744

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Mouse Group IID Secretory Phospholipase A2 (Pla2g2d), N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 15.7 kDa. The purity is> 97%.

Specifications

Type Recombinant Protein
Species Mouse
Expression System E.coli
Purity > 97%
Endotoxin <1.0 EU per 1µg (determined by the LAL method)
Predicted Molecular Weight 15.7 kDa
Physical State Lyophilized
Formulation 100 mMNaHCO3, 500 mMNaCl, pH8.3, containing 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Target Information

Mouse Group IID Secretory Phospholipase A2 (Pla2g2d) is a calcium-dependent enzyme that hydrolyzes the sn-2 fatty acid ester bond of glycerophospholipids, producing free fatty acids and lysophospholipids. It is highly expressed in dendritic cells and plays a crucial role in immune regulation. Pla2g2d has anti-inflammatory and immunosuppressive functions, contributing to the resolution of Th1 immune responses by controlling the levels of anti-inflammatory lipids such as docosahexaenoic acid and its metabolites. In mouse models, Pla2g2d deficiency exacerbates psoriasis and contact dermatitis, while overexpression ameliorates these conditions. Additionally, Pla2g2d has bactericidal activity against Gram-positive bacteria by directly hydrolyzing bacterial membrane phospholipids.

Protein Mouse Group IID Secretory Phospholipase A2 (Pla2g2d)
Protein Synonym GIID sPLA2; sPLA2-IID
Gene ID 18782
EC No. Q9WVF6

Shipping and Storage

This product is shipped with ice packs. Lyophilized protein can be stored at -80°C for 1 year. After reconstitution, the protein solution can be stored at 2-8°C for 2-7 days.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 100 mM NaHCO3, 500 mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimize freeze-thaw cycles.
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