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Mouse Hedgehog Interacting Protein (HHIP) ELISA Kit-Sandwich

Cat. No.EK6F177

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Hedgehog Interacting Protein (HHIP) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse HHIP in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Hedgehog Interacting Protein (HHIP).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Hedgehog-interacting protein (HIP) modulates Hedgehog signaling in various cell types, including those in the brain and lungs, through direct interaction with Hedgehog family members. In its soluble form, HIP inhibits Sonic Hedgehog (Shh)-induced differentiation in the fibroblast cell line C3H/10T1/2. In mice, HIP's primary role is to inhibit the Hedgehog signaling pathway by competitively binding with the Hedgehog ligand, Ptch-1. This regulation affects processes such as cell fate determination, tissue patterning, and disease development. In mouse embryos, HIP is expressed in cells located near Shh-expressing cells, indicating that HIP may inhibit the Hedgehog signaling pathway by competing for binding with the Shh ligand, Ptch-1.

Target/Biomarker Mouse HHIP
Target Synonym HIP
Gene ID 15245
UniProt ID Q7TN16

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Olsen, Catherine L et al. "Hedgehog-interacting protein is highly expressed in endothelial cells but down-regulated during angiogenesis and in several human tumors." BMC cancer vol. 4 43. 4 Aug. 2004.
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