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Mouse Deiodinase, Iodothyronine, Type II (DIO2) ELISA Kit-Sandwich

Cat. No.EK6F335

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Deiodinase, Iodothyronine, Type II (DIO2) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse DIO2 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Deiodinase, Iodothyronine, Type II (DIO2).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.8-50 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Type II iodothyronine deiodinase is essential in thyroid hormone metabolism, particularly in the activation and inactivation of these hormones through deiodination. It catalyzes the conversion of T4 (L-thyroxine/3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and rT3 (3,3',5'-triiodothyronine) into T2 (3,3'-diiodothyronine) via outer-ring deiodination (ORD). Dio2 is chiefly responsible for converting T4 to T3, a vital step in sustaining thyroid hormone balance. Mice deficient in Dio2 (D2KO) display thyroid dysfunction, including elevated TSH levels and increased tolerance to T4, while maintaining normal T3 levels. Furthermore, Dio2 facilitates cold-adaptive thermogenesis in brown adipose tissue by regulating energy metabolism through enhanced T3 production.

Target/Biomarker Mouse DIO2
Target Synonym 5DII; D2; SelY; TXDI2; Thyroxine Deiodinase;Type II; Type-II 5'-deiodinase
Gene ID 13371
UniProt ID Q9Z1Y9

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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