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Mouse Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) ELISA Kit-Sandwich

Cat. No.EK5F282

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse CDKN2A in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Cyclin Dependent Kinase Inhibitor 2A (CDKN2A).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.32-20 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Cyclin-dependent kinase inhibitor 2A (Cdkn2a) functions as a negative regulator of normal cell proliferation by strongly interacting with CDK4 and CDK6, thereby inhibiting their interaction with cyclins D and their ability to phosphorylate the retinoblastoma protein. In mice, Cdkn2a is extensively involved in cell proliferation, differentiation, metabolic regulation, tumor suppression, cardiac repair, and tissue aging. Together with p16Ink4a and p14Arf, Cdkn2a forms a vital tumor suppressor gene cluster. Research has shown that the loss of Cdkn2a facilitates tumor development and progression. For instance, in a mouse model, the simultaneous loss of Cdkn2a and Arf accelerates asbestos-induced mesothelioma, mirroring the molecular characteristics of human malignant mesothelioma.

Target/Biomarker Mouse CDKN2A
Target Synonym P16; ARF; CDK4I; CMM2; INK4; INK4a; MLM; MTS1; TP16; P14; P14ARF; P16INK4a; P19; Melanoma P16; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1
Gene ID 12578
UniProt ID P51480

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Altomare, Deborah A et al. "Losses of both products of the Cdkn2a/Arf locus contribute to asbestos-induced mesothelioma development and cooperate to accelerate tumorigenesis." PloS one vol. 6,4 e18828. 19 Apr. 2011.
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