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Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit-Sandwich

Cat. No.EK6F590

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse CH25H in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Cholesterol-25-Hydroxylase (CH25H).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.8-50 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Cholesterol 25-hydroxylase catalyzes the conversion of cholesterol to 25-hydroxycholesterol, which suppresses cholesterol biosynthetic enzymes. It plays a crucial role in cell positioning and movement within lymphoid tissues, as 25-hydroxycholesterol is an intermediate in the synthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC). This oxysterol serves as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for various lymphoid cells. In Alzheimer's disease (AD) mouse models, increased expression of CH25H is closely linked to neuroinflammation and neurodegenerative changes. Research indicates that CH25H can exacerbate neuroinflammation and amyloid deposition by amplifying inflammatory signals. However, in some instances, CH25H might also reduce inflammatory responses by inhibiting SREBP processing.

Target/Biomarker Mouse CH25H
Target Synonym h25OH; Cholesterol 25-monooxygenase
Gene ID 12642
UniProt ID Q9Z0F5

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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