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Mouse Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) ELISA Kit-Sandwich

Cat. No.EK6F476

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse SPINK5 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Serine Peptidase Inhibitor Kazal Type 5 (SPINK5).
Target Species Mouse
Species Reactivity Mouse
Detection Range 78.25-5000 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

SPINK5 encodes the protease inhibitor LEKTI, primarily responsible for inhibiting serine proteases in the epidermis, such as KLK5 and KLK7, to maintain skin barrier integrity. LEKTI binds to serine proteases, preventing their overactivation and thereby avoiding excessive keratinocyte shedding and damage to the skin barrier. In mice, the SPINK5 gene plays a crucial role in skin barrier function, keratinocyte differentiation, and protease activity regulation. These roles have been confirmed in various disease models, particularly in genetic conditions associated with Netherton syndrome (NS).

Target/Biomarker Mouse SPINK5
Target Synonym LEKTI; LETKI; NETS; NS; VAKTI; Lymphoepithelial Kazal-Type-Related Inhibitor; Lympho-epithelial Kazal-type-related inhibitor

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Furio, Laetitia et al. "KLK5 Inactivation Reverses Cutaneous Hallmarks of Netherton Syndrome." PLoS genetics vol. 11,9 e1005389. 21 Sep. 2015.
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