Veterinary Antimicrobial Resistance (AMR) Gene Discovery

Antimicrobials are essential tools in treating infectious diseases in animals, but their misuse and overuse have led to the emergence of resistant strains of bacteria, fungal. Antimicrobial resistance makes standard treatments ineffective, resulting in persistent infections and a higher risk of transmission of resistant pathogens. Understanding and discovering AMR genes in veterinary settings is crucial for developing pathogen infection treatment among animals. BioVenic has established advanced molecular analysis platforms, providing specialized service for the discovery of unknown new drug resistance genes and gene variants in animal-associated disease-causing pathogens.

Genetic Resistance Mechanism

Pathogens often develop drug resistance through various genetic mechanisms, such as horizontal gene transfer and mutations. The acquisition of foreign DNA via transformation, phage-mediated transduction, and conjugation is a major driver of bacterial evolution and often leads to antimicrobial resistance. Additionally, pathogens can acquire mutations in genes that affect drug uptake, efflux, inactivation, and target modification, allowing them to survive despite the presence of antimicrobial agents.

Veterinary AMR Gene Discovery Services

Using state-of-the-art equipment and extensive knowledge in molecular biology and microbiology, our veterinary services for antimicrobial resistance genotypic characterization employ various molecular techniques. These services identify genes linked to antimicrobial resistance in animal pathogens and uncover the mechanisms behind this resistance.

• Whole Genome Sequencing
  Whole Genome Sequencing (WGS) is a powerful tool that enables the comprehensive analysis of pathogens' complete genetic material. Our WGS service is particularly valuable in the study of antimicrobial resistance (AMR) as it allows for the identification of novel resistance genes within a pathogens' genome, aiding in antimicrobial resistance molecular mechanism studies.
• De novo Sequencing
  De Novo sequencing is a method used to assemble genomes from scratch without the need for a reference genome. BioVenic provide trusted de novo genome sequencing service. Our service includes construction of the entire genome sequence of a pathogen from fragmented DNA sequences, providing high-resolution and accurate genetic maps. Our service is essential for identifying subtle genetic variations and complex genomic structures in pathogen antimicrobial resistance and novel drug target discovery.

Fig.2 Schematic of AMR surveillance using WGS and WMS^1,2.

Service Workflow

By providing cutting-edge molecular research services, BioVenic aims to uncover new drug resistance genes and gene variants, enhancing our ability to combat antimicrobial resistance in animal-associated pathogens. Our expertise in gene function analysis, mutation studies, transcriptomics, and resistance mechanisms positions us at the forefront of this critical field. Please feel free to contact us to learn more about our AMR gene discovery services and advance your research and develop strategies to safeguard animal health against the threat of antimicrobial resistance.

References

1. Oniciuc, Elena A., et al. "The present and future of whole genome sequencing (WGS) and whole metagenome sequencing (WMS) for surveillance of antimicrobial resistant microorganisms and antimicrobial resistance genes across the food chain." Genes 9.5 (2018): 268.
2. Image retrieved from Figure "Graphical Abstract", Oniciuc, Elena A., et al., 2018, used under CC BY 4.0. The original image was modified by changing the title to "Schematic of AMR surveillance using WGS and WMS".