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Veterinary Antimicrobial Resistance (AMR) Gene Discovery
BioVenic operates advanced molecular analysis platforms and delivers specialized services to identify unknown novel antimicrobial resistance genes and gene variants within animal pathogenic microorganisms. Antimicrobials serve as vital tools for treating animal infectious diseases. However, their misuse and overuse have triggered the emergence of resistant bacterial and fungal strains. Antimicrobial resistance renders conventional therapies ineffective, leading to persistent infections and an elevated risk of transmission of resistant pathogens. Identifying and characterizing AMR genes within veterinary contexts is critical for advancing therapeutic strategies against animal pathogen infections.
Genetic Resistance Mechanism
Pathogens often develop drug resistance through various genetic mechanisms, such as horizontal gene transfer and mutations. The acquisition of foreign DNA via transformation, phage-mediated transduction, and conjugation is a major driver of bacterial evolution and often leads to antimicrobial resistance. Additionally, pathogens can acquire mutations in genes that affect drug uptake, efflux, inactivation, and target modification, allowing them to survive despite the presence of antimicrobial agents.
Veterinary AMR Gene Discovery Services
Using state-of-the-art equipment and extensive knowledge in molecular biology and microbiology, our veterinary services for antimicrobial resistance genotypic characterization employ various molecular techniques. These services identify genes linked to antimicrobial resistance in animal pathogens and uncover the mechanisms behind this resistance.
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Whole Genome Sequencing
Whole Genome Sequencing (WGS) is a powerful tool that enables the comprehensive analysis of pathogens' complete genetic material. Our WGS service is particularly valuable in the study of antimicrobial resistance (AMR) as it allows for the identification of novel resistance genes within a pathogens' genome, aiding in antimicrobial resistance molecular mechanism studies. -
De novo Sequencing
De Novo sequencing is a method used to assemble genomes from scratch without the need for a reference genome. BioVenic provide trusted de novo genome sequencing service. Our service includes construction of the entire genome sequence of a pathogen from fragmented DNA sequences, providing high-resolution and accurate genetic maps. Our service is essential for identifying subtle genetic variations and complex genomic structures in pathogen antimicrobial resistance and novel drug target discovery.
Fig.2 Schematic of AMR surveillance using WGS and WMS1,2.
Service Workflow
Frequently Asked Questions
What core sequencing technologies support your veterinary AMR novel gene mining work?
We offer two core sequencing solutions for resistance gene discovery: whole genome sequencing for known veterinary pathogens to screen resistance gene variants, and de novo whole-genome assembly for novel, unannotated resistant strains without reference genomes, enabling full excavation of unknown AMR genes and special structural variations linked to drug tolerance.
What major genetic pathways do pathogens rely on to generate antimicrobial resistance?
Pathogens gain drug resistance via two primary genetic routes: horizontal gene transfer including transformation, phage transduction and bacterial conjugation to acquire exogenous resistance fragments. And spontaneous gene mutations that alter drug intake, efflux efficiency, target protein structure or drug inactivation capacity of pathogens.
What research values does AMR gene discovery bring to veterinary infectious disease control?
Uncovering novel resistance genes and their molecular mechanisms helps explain failed clinical antimicrobial treatments, tracks the transmission of resistant bacteria along the breeding and food chain, provides gene targets for developing rapid AMR molecular diagnostic reagents, and guides scientific, reduced antimicrobial usage strategies for livestock farms.
What supporting analytical capabilities do you match with AMR gene sequencing services?
Beyond genome assembly and resistance gene annotation, we supply supporting multi-omics analysis including pathogen transcriptomics for exploring differential expression of resistance genes under drug stress, mutation site verification, and comparative genomics to trace the horizontal transfer route of resistance gene fragments between strains.
What types of animal pathogen samples are applicable to your AMR gene discovery platform?
Our platform accepts pure cultured resistant bacterial and fungal strains isolated from livestock, poultry and companion animals, as well as mixed pathogen samples from breeding environments, feces and diseased animal tissues, supporting systematic AMR gene mining for single strains and complex flora communities alike.
Partner with BioVenic
By providing cutting-edge molecular research services, BioVenic aims to uncover new drug resistance genes and gene variants, enhancing our ability to combat antimicrobial resistance in animal-associated pathogens. Our expertise in gene function analysis, mutation studies, transcriptomics, and resistance mechanisms positions us at the forefront of this critical field. Please feel free to contact us to learn more about our AMR gene discovery services and advance your research and develop strategies to safeguard animal health against the threat of antimicrobial resistance.
References
- Oniciuc, Elena A., et al. "The present and future of whole genome sequencing (WGS) and whole metagenome sequencing (WMS) for surveillance of antimicrobial resistant microorganisms and antimicrobial resistance genes across the food chain." Genes 9.5 (2018): 268.
- Image retrieved from Figure "Graphical Abstract", Oniciuc, Elena A., et al., 2018, used under CC BY 4.0. The original image was modified by changing the title to "Schematic of AMR surveillance using WGS and WMS".