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Porcine Dihydrofolate Reductase (DHFR) ELISA Kit-Sandwich

Cat. No.EK12F644

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Porcine Dihydrofolate Reductase (DHFR) ELISA Kit-Sandwich is designed for the quantitative determination of Porcine Dihydrofolate Reductase (DHFR) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Porcine DHFR.
Target Species Swine
Species Reactivity Swine
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%
Assay Time Around 270 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

Dihydrofolate reductase (DHFR) is an enzyme that plays a critical role in cellular processes by reducing dihydrofolic acid to tetrahydrofolic acid. Dihydrofolate Reductase is encoded by the DHFR gene in pigs. Dihydrofolate reductases from different species can be distinguished by their pattern of binding of diaminoheterocyclic molecules. The amino acid sequence of porcine liver dihydrofolate reductase has been shown to be very similar to that of the L1210 enzyme.

Target/Biomarker Porcine DHFR
Target Synonym Dihydrofolate reductase; DHFR; 1.5.1.3
Gene ID 100525912
UniProt ID P00377

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Smith, S. L. et al. Porcine liver dihydrofolate reductase. Purification, properties, and amino acid sequence. The Journal of biological chemistry. 1979, 254: 11475-11484.
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