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Gene Knock-in in Precision Animal Breeding
Based on its extensive experience in animal genome editing projects and an exceptional molecular biology experimental team, BioVenic has introduced personalized genome knock-in services to customers to address various challenges in transgene knock-in for precision animal breeding research.
Background of Knock-in
BioVenic delivers customized genome knock-in services to tackle diverse obstacles related to transgene knock-in within precision animal breeding research. Backed by rich experience in animal genome editing projects and a professional molecular biology experimental team, we provide reliable technical support to meet varied research demands.
Fig.1 Schematic Diagram of Knock-in System. (BioVenic Original)
Our Services
Applications of Gene Knock-in in Animal Breeding
The application of knock-in editing in precision animal breeding is also quite extensive. Researchers obtain better strains by knocking in to inactivate the target gene or directly knocking in the entire exogenous functional gene. Examples of animal breeding using knock-in (KI) include the mouse-derived UCP1 KI pigs with cold resistance improvement, and goats with meat quality improvement by knocking out the MSTN gene and inserting the exogenous fat-1 gene specifically. Gene knock-in technology creates new breeds of economically valuable farmed animals by breaking species boundaries.
Why Choose Us?
Stable expression of foreign functional genes after insertion.
High-precision seamless knock-in.
No adverse effects on the animal genome.
Related Research
Published work demonstrated a site-specific gene knock-in strategy targeting the ovine Rosa26 safe-harbour genomic locus. Schematic layouts described a donor-vector construct equipped with 1.0-kb bilateral homology arms flanking a CAG-driven tGFP reporter cassette for site-directed cassette integration. Follow-up cellular assays further validated obvious reporter fluorescence signal in sheep fibroblasts after construct delivery, verifying that this donor template can support stable exogenous reporter gene expression upon accurate targeted insertion at this safe-harbour site.
Drawing on such safe-harbour-oriented gene knock-in research outcomes, BioVenic carries out broad-scale exploration of technical solutions for precision animal breeding.
Fig.1 Diagram of knock-in of LA-CAG-tGFP-polyA-RA into the sRosa26 locus.1
Frequently Asked Questions
What full modules does your site-specific gene knock-in one-stop service cover?
We supply four core technical services: customized construction of gene editing and donor knock-in vectors, optimized design and synthesis of guide RNA and HDR homologous repair templates, complete experimental scheme customization for farm animal genome knock-in, and high-throughput sequencing-based verification of genomic modification results after editing.
What technical advantages does homologous recombination knock-in have over random transgene insertion?
Targeted HDR knock-in avoids positional interference, gene silencing and damage to adjacent genomic loci caused by random insertion. It realizes seamless fixed-site integration of exogenous gene fragments, ensures stable long-term expression of inserted functional genes, and brings no extra adverse impacts on the host animal genome.
What practical breeding research scenarios can gene knock-in technology support?
It enables directional cultivation of superior livestock germplasm, such as knocking in UCP1 gene to enhance cold resistance and reduce fat deposition in pigs, inserting fat-1 gene at MSTN locus of goats to improve meat quality, and introducing functional exogenous genes across species to create new high-economic-value farm animal strains.
How do you optimize editing efficiency during knock-in project execution?
We optimize guide RNA targeting specificity and homologous arm sequences of HDR templates via professional bioinformatics prediction. Matching customized vector construction and transfection schemes are adopted to elevate homologous recombination efficiency and raise the positive rate of site-specific knock-in animal cell lines or embryos.
What verification methods do you use to confirm successful genome knock-in?
We apply high-throughput sequencing technology for full-site detection of edited animal genomes, precisely verify whether the exogenous fragment is seamlessly integrated at the preset target locus, check the integrity of inserted gene sequences, and exclude off-target random insertion to guarantee reliable editing identification results.
Partner with BioVenic
Relying on extensive experience in scientific research projects, mature technical support, and guidance, BioVenic offers professional and efficient one-stop customized services for your knock-in requirements, providing comprehensive and precise knock-in solutions that are tailored to meet your specific breeding research needs. Feel free to contact us for any additional information or pricing inquiries.
Reference
- Wu, Mingming, et al. "Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system." Scientific Reports 6.1 (2016): 24360. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.1038/srep24360