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Recombinant Bovine Antithrombin-III (SERPINC1)
Product Overview
BioVenic's Recombinant Bovine Antithrombin-III (SERPINC1) is a recombinant protein expressed from E.coli. Its predicted molecular weight is N/A. The purity is>97% (SDS-PAGE).
Specifications
Target Information
Bovine antithrombin-III (ATIII) is a plasma glycoprotein that is a key inhibitor in the blood coagulation cascade. It is primarily synthesized in the liver and has a normal plasma concentration of approximately 2 μM. ATIII inhibits serine proteases of the intrinsic coagulation pathway such as Factor IXa, Xa, and thrombin, which is its principal physiological target. The inhibition of thrombin by ATIII occurs through the formation of a stable equimolar complex between the inhibitor and protease, which can be enhanced by catalytic amounts of heparin, a polysaccharide. This process is essential for preventing excessive blood clotting and maintaining blood fluidity.
Shipping and Storage
This product is shipped with dry ice. It is recommended to aliquot as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months. Avoid repeated freezing and thawing.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimise freeze-thaw cycles.
References
- Stern, D. et al. Interaction of antithrombin III with Sheep aortic segments. Role of heparin in binding and enhanced anticoagulant activity. The Journal of clinical investigation. 1985, 75,1: 272-9.