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Chemically-induced Animal Model Development Service
Introduction
Chemically induced animal models are a vital and indispensable tool in modern biomedical research. BioVenic provides chemically induced animal model development services, which assist researchers in the chemical modeling and subsequent study of various diseases in multiple animal species.
BioVenic Chemically-induced Animal Model Development Services
Establishment and Validation of Chemically-Induced Animal Models
BioVenic's chemically-induced animal model development services allow for the comprehensive selection of the appropriate animal species, inducer, and administration method, tailored to the disease type and research objectives required by the client. For the chemically-induced animal models we generate, we are able to perform multi-dimensional validation using a range of assessment methods, including behavioral analysis, blood biochemistry, histology, molecular biology, and imaging techniques. This ensures that the models are consistent with the human disease in terms of phenotype, pathology, and underlying mechanism.
Table. 1 BioVenic's Multi-dimensional Validation for Chemically-induced Animal Models.
| Behavioral Assessment | Histological/Pathological Examination | Biochemical and Molecular Biology Assessment | Pharmacological Validation |
|---|---|---|---|
| Observing animal behaviors that are associated with the symptoms of the modeled disease (e.g., locomotor activity, changes in body weight, blood glucose levels, seizure scores, etc.). | Employing methods such as H&E staining and immunohistochemistry (IHC) to verify pathological changes (e.g., loss of dopaminergic neurons in substantia nigra, levels of liver fibrosis, etc.). | Using methods such as ELISA and qPCR to quantify specific molecules in sera or tissues (e.g., TNF-alpha, IL-6, TGF-beta, insulin, etc.). | Administering clinically known effective drugs to observe whether they can reverse or alleviate the model's manifestations. |
Sample Collection Services for Chemically-induced Animal Models
After creating and testing chemically-induced animal models, the BioVenic team offers full bio-sample collection services for these models during studies that use them. We can quickly get samples of body fluids (like blood, urine, and cerebrospinal fluid) and tissues, and we can also do the right processing (like DNA and RNA extraction) to give researchers the materials they need for their experiments. Alternatively, our team can analyze these bio-samples to provide data relevant to the required target indicators.
Research Services Conducted on Chemically-induced Animal Models
BioVenic offers non-GLP preclinical research services specifically tailored for chemically-induced animal models. Utilizing the established animal models, the BioVenic team can provide integrated research and analysis services across your desired areas of study, including:
- Animal Behavioral Analysis
- Animal Histopathology Service
- Preclinical Animal Pharmacodynamics (PD) Study
- Preclinical Animal Pharmacokinetics (PK) Study
- Animal Cell Biology Service
- Animal lmaging Service
Explore other BioVenic animal model development services:
- Genome-induced Animal Model Development
- Diet-induced Animal Model Development
- Surgically-induced Animal Model Development
- Biologically-induced Animal Model Development
Typical Development Workflow for a Chemically-induced Animal Model
In the preparation and execution of BioVenic's experimental protocol for constructing chemically induced animal models, our team follow a rigorous development process. The following standardized workflow outlines the systematic approach for establishing and comprehensively validating chemically induced animal models for preclinical research at BioVenic.
Fig. 1 Development Workflow for a Chemically-induced Animal Model (BioVenic Original)
Administration Methods for Chemically-induced Animal Model Development
The BioVenic team is capable of assisting in the selection of the most appropriate method of administration of the chemical inducer by taking into account a number of factors, including the physicochemical properties of the chemical agent (solubility in water, volatility, local irritating properties), the availability of the target tissue (blood-brain barrier, joint cavity, mammary ducts), as well as the model requirements (animal model, the need to administer a continuous low dose or a high one-time dose).
Tabel. 2 Administration Methods for Chemically-induced Animal Model Development
| Systemic Administration | Local Administration |
|---|---|
| Oral, intraperitoneal (IP), intravenous (IV), subcutaneous (SC)/intramuscular (IM). | Rectal, colon catheter, joint cavity, mammary ducts, nasal cavity, intracerebral. |
| Suitable for chemical agents that require whole-body exposure, such as STZ, DSS, TNBS, and nitroglycerin. | Allows for high-concentration induction with low systemic side effects within a specific tissue or organ, and is commonly used for models of inflammation, pain, tumors, or neurotoxicity. |
Common Applications of Chemically-induced Animal Models
BioVenic's platform has the capability to establish different kinds of animal models by chemical induction and can be applied in many different fields, such as cancer, digestive system diseases, metabolic diseases, nervous system diseases, and bone and joint diseases.
Table. 3 The Most Frequently Used Chemically-Induced Animal Models and Their Induction Mechanisms
| Disease Type | Model Example | Common Chemical Inducers | Mechanism Summary | Common Animal Types |
|---|---|---|---|---|
| Cancer | Liver Cancer, Colorectal Cancer, Lung Cancer, etc. | Diethylnitrosamine (DEN), Azoxymethane (AOM), Methylnitrosourea (MNU) | Induces DNA damage, initiating cell malignant transformation and carcinogenesis. | Mouse, Rat |
| Inflammatory Bowel Disease (IBD) | Colitis | Dextran Sodium Sulfate (DSS), Trinitrobenzenesulfonic acid (TNBS), Oxazolone | Damages the intestinal epithelial barrier, leading to exposure to gut microbes and subsequent immune-mediated inflammation. | Mouse |
| Metabolic Disease | Type 1 / Type 2 Diabetes | Streptozotocin (STZ) - common for Type 1; High-Fat Diet / STZ - common for Type 2 | Selectively destroys pancreatic Beta cells, leading to insulin deficiency or insulin resistance. | Mouse, Rat |
| Liver Injury/Fibrosis | Liver Fibrosis, Cirrhosis | Carbon Tetrachloride (CCl4), Thioacetamide (TAA) | Generates free radicals and reactive metabolites, causing toxic damage to hepatocytes and subsequent reparative fibrosis. | Mouse, Rat |
| Neurodegenerative Disease | Parkinson's Disease | 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 6-Hydroxydopamine (6-OHDA) | Selectively destroys dopaminergic neurons in the substantia nigra of the brain, mimicking the motor symptoms of Parkinson's disease. | Mouse, Rat, Non-Human Primate |
| Chronic Pain | Inflammatory Pain, Neuropathic Pain | Complete Freund's Adjuvant (CFA), Carrageenan, Lipopolysaccharide (LPS) | Induces local or systemic inflammatory responses, stimulating nociceptors or causing peripheral nerve damage. | Mouse, Rat |
Fig. 2 Diabetes Induction Model with STZ.1,3
Case Study: Standardized Chemically Induced Colitis Models for Preclinical Screening
In order to conduct drug efficacy studies on a large scale for Inflammatory Bowel Disease (IBD) treatments, BioVenic offers a standardized DSS-induced colitis model that has been optimized for maximum reproducibility and efficiency. We specialize in the essential key parameters that are necessary for pharmaceutical benchmarking. We assure robust data for your preclinical screening needs. We include a full spectrum of monitoring for exact daily measurements for body weight loss, stool consistency, and occult blood. In our validation package, we include the most clinically relevant measures for Inflammatory Bowel Disease. These include morphological evaluation for colon shortening and histological evaluation for mucosal integrity using a semi-quantitative histological scoring system for H&E staining. In addition, we include a wide range of bioanalytical services that include the quantification of key pro-inflammatory cytokines like TNF-alpha, IL-1-beta, IL-6 using ELISA technology, along with MPO activity. We include these industry-standard clinical, morphological, and molecular readouts for our chemical induction services that help evaluate the therapeutic potential for new anti-inflammatory and immunomodulatory agents.
Fig 3. Macro- and Micro-scopic Assessment of DSS-induced Colitis Animal Colon.2,3
Advantages of BioVenic Chemically-induced Animal Model Development Service
Highly Customized Solutions
Our experts at BioVenic conduct comprehensive needs assessment based on the project's research goals and will provide help in the tailored design of key parameters such as inducer type, dosage, administration method, and experimental time points, ensuring the study protocol is highly aligned with the research objective.
Extensive Animal and Cell Model Library
BioVenic has a variety of commonly used animal models (e.g., mice, rats, etc.), which are accompanied by corresponding cell lines (primary cells, stable transfection cells). This flexibility is advantageous for the selection of the appropriate animal model or specific physiological state for the disease condition under study.
Specialized Expertise and Advanced Facilities
BioVenic possesses deep scientific expertise across chemically-induced animal model development areas and invest in state-of-the-art, accredited facilities. With our integrated platform, scientists can conduct research on multiple aspects of the development and experimentation of chemically induced animal models.
FAQs
Do you provide live model animals?
Currently, we do not provide live animals. Instead, we offer one-stop, non-GLP preclinical services based on chemically-induced animal models. Once the model is established successfully, you may choose to continue to use our platform for a variety of downstream research activities such as drug administration, testing for efficacy indicators, analysis of animal tissues/body fluids, pathological analysis, as well as pharmacokinetic studies to further enhance your research efficiency.
What are the advantages of chemically-induced methods over other methods in animal model development, and can you provide an example?
Chemically-induced animal models are advantageous over the genetic or transplant-based approach in that they reflect the natural progression of the disease process in the case of cancer or inflammation. The chemically-induced approach is also a quicker, more cost-effective, and less labor-intensive approach compared to the genetically-engineered approach. A precise temporal control of the disease onset is also another advantage. For example, the administration of the chemical compound DSS in the drinking water of mice results in the induction of colitis. This allows for the easy control of the disease process in the mouse model of colitis that is relevant to human Inflammatory Bowel Disease.
What kind of data will you provide to confirm the successful establishment of the chemically-induced model?
Based on the client's needs, our delivered reports include comprehensive validation data, such as: 1. Histopathology (e.g., H&E, special staining); 2. Biochemical indicators in blood, tissue, and other samples; 3. qPCR or Western Blot results for key molecular markers; and 4. Animal physiological indicators (body weight, food intake, behavioral scoring, etc.).
What happens if the model induction fails or the effect is not ideal?
We conduct a rigorous evaluation of your requirements before starting the study. If the model fails to meet the validation standards stipulated in the contract, we will rebuild the model. If we are still unable to provide an ideal research model and subsequent research project, we will provide the stage-specific results achieved and adjust the fee after negotiation with you, in order to protect the client's research progress.
Partner with Us
BioVenic's services comprehensively cover the establishment and validation of chemically induced animal models, sample collection from the models, preclinical non-GLP animal testing and research, as well as the detection of relevant behavioral, physiological/biochemical, and pathological indicators. If you have a need for chemically induced animal model development services, please feel free to contact us with your requirements. Our technical team will comprehensively evaluate your needs and will be happy to provide subsequent services.
References
- Wszola, Michal et al. "Streptozotocin-Induced Diabetes in a Mouse Model (BALB/c) Is Not an Effective Model for Research on Transplantation Procedures in the Treatment of Type 1 Diabetes." Biomedicines vol. 9,12 1790. 29 Nov. 2021, https://doi.org/10.3390/biomedicines9121790. Without modification.
- López-Estévez, Sergio et al. "Long-lasting visceral hypersensitivity in a model of DSS-induced colitis in rats." Neurogastroenterology and motility vol. 34,11 (2022): e14441. https://doi.org/10.1111/nmo.14441. The original title was changed to "Macro- and Micro-scopic Assessment of DSS-induced Colitis Animal Colon."
- Distributed under Open Access license CC BY 4.0.