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Mouse Succinate Dehydrogenase Complex Subunit A (SDHA) ELISA Kit-Sandwich

Cat. No.EK6F494

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Succinate Dehydrogenase Complex Subunit A (SDHA) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse SDHA in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Succinate Dehydrogenase Complex Subunit A (SDHA).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.32-20 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

The succinate dehydrogenase [ubiquinone] flavoprotein subunit, located in the mitochondria, is a flavoprotein (FP) component of succinate dehydrogenase (SDH) within complex II of the mitochondrial electron transport chain. It plays a crucial role in transferring electrons from succinate to ubiquinone (coenzyme Q). In mice, knocking out the Sdha gene results in mild muscle fiber atrophy and mild behavioral or biochemical abnormalities. Sdha is also key in regulating cellular metabolic pathways. In a non-alcoholic fatty liver disease (NAFLD) model, the expression level of Sdha is closely linked to the liver's energy metabolism state. In addition, in the mouse brain, the activity and expression level of Sdha are generally higher in females than males.

Target/Biomarker Mouse SDHA
Target Synonym SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial
Gene ID 66945
UniProt ID Q8K2B3

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Zaręba-Kozioł, Monika et al. "S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity." International journal of molecular sciences vol. 22,12 6253. 10 Jun. 2021.
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