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Mouse Crystallin Beta A1 (CRYbA1) ELISA Kit-Sandwich

Cat. No.EK6F542

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Crystallin Beta A1 (CRYbA1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse CRYbA1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Crystallin Beta A1 (CRYbA1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.32-20 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Crystallins are the primary structural components of the vertebrate eye lens. In mouse retinal astrocytes, βA1-crystallin mainly functions to regulate glucose metabolism and mitochondrial function. This regulation is achieved by modulating the activity of protein tyrosine phosphatase 1B (PTP1B). βA1-crystallin binds to PTP1B and inhibits its phosphatase activity through an allosteric site, rather than the active site, ensuring normal glucose metabolism. Under conditions of hyperglycemic stress, the absence of βA1-crystallin results in increased PTP1B activity, which in turn inhibits the STAT3 signaling pathway. This leads to disturbances in glucose metabolism, heightened oxidative stress, and an inflammatory response.

Target/Biomarker Mouse CRYbA1
Target Synonym CRYB1; Eye Lens Structural Protein
UniProt ID P02525

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Ghosh, Sayan et al. "βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity." Communications biology vol. 4,1 248. 24 Feb. 2021.
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