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Recombinant Mouse Hypoxanthine-Guanine Phosphoribosyltransferase (Hprt1), N-His-GST

Cat. No.AP4C305

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Mouse Hypoxanthine-Guanine Phosphoribosyltransferase (Hprt1), N-His-GST is a recombinant protein expressed from E.coli. Its predicted molecular weight is 54.3 kDa. The purity is> 95%.

Specifications

Type Recombinant Protein
Species Mouse
Expression System E.coli
Purity > 95%
Endotoxin < 1.0 EU/μg
Predicted Molecular Weight 54.3 kDa
Physical State Lyophilized
Formulation 20 mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Target Information

Mouse Hypoxanthine-Guanine Phosphoribosyltransferase (Hprt1) is a key enzyme in the purine salvage pathway, essential for recycling purine bases and maintaining cellular purine levels. It is involved in the conversion of hypoxanthine to inosine monophosphate (IMP) and guanine to guanosine monophosphate (GMP). Hprt1 deficiency is associated with Lesch-Nyhan syndrome, characterized by hyperuricemia, neurological dysfunction, and self-injurious behavior. It also plays roles in cancer progression, particularly in head and neck squamous cell carcinoma (HNSCC), where its upregulation correlates with advanced tumor progression and poor prognosis. Hprt1 is a potential therapeutic target in metabolic and neurological disorders as well as in cancer.

Protein Mouse Hypoxanthine-Guanine Phosphoribosyltransferase (Hprt1)
Protein Synonym HPRT B; HGPRT; HGPRTase
Gene ID 15452
EC No. P00493

Shipping and Storage

This product is shipped with ice packs. Lyophilized protein can be stored at -80°C for 1 year. After reconstitution, the protein solution can be stored at 2-8°C for 2-7 days.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 20 mM Tris, 150 mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimize freeze-thaw cycles.
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