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Mouse Bladder Cancer Cells-MB49-Luc-Puro

Cat. No.CC8F52

Product TypeAnimal Cells and Cell Lines

Product Overview

MB49 cells are an immortalized mouse bladder cancer cell line derived from C57BL/Icrf-a mouse bladder epithelial cells. These cells were transformed after a single 24-hour treatment with the chemical carcinogen 7,12-dimethylbenzanthracene (DMBA) during long-term primary culture. The transformed cells, when transplanted into syngeneic mice, were shown to produce carcinomas. Although derived from a male, karyotype analysis revealed a 100% loss of the Y chromosome in the cells analyzed. Mouse bladder cancer cells-MB49-Luc-Puro were obtained by lentiviral infection of MB49 cells, resulting in stable expression of firefly luciferase.

Specifications

Cell Product Type Cell Line
Species Mouse
Cell Type Tumor Cell Line
Source Bladder Cancer
Disease Bladder Cancer
Growth Information Adherent; 37°C; 5%CO2
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Formulation and Cell Culture Medium DMEM high glucose (with sodium pyruvate), containing 10% FBS
Physical State Frozen cells

Target Information

This cell line is a cell line stably expressing firefly luciferase obtained by infecting MB49 cells with lentivirus.

Target/Biomarker Luciferase
Target Synonym luciferin 4-monooxygenase [ Photinus pyralis (common eastern firefly) ]; luc; LUC1; PpyLuc1
Gene ID 116160065

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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