Indel Detection by Amplicon Analysis (IDAA) Assay

Indel detection by amplicon analysis (IDAA) assay is an outstanding method for the identification of insertions and deletions (indels) induced by precise gene editing. BioVenic relies on a professional team of experts to provide quality services based on indel detection by amplicon analysis assay. Our scientists are proud to be able to provide our customers with information and advice on the field of gene editing validation with our maximum knowledge and competence.

Indel Detection by Amplicon Analysis Assay for Gene Editing Validation

Gene-targeting technologies for precise editing of higher eukaryotic genomes, such as zinc finger nucleases (ZFNs), RNA-guided clustered regularly interspaced short palindromic repeats (CRISPRs), transcription activator-like effector nucleases (TALENs), or meganucleases, are revolutionizing traditional genome research. A primary limitation of precision gene editing applications is the lack of efficient and sensitive methods to detect and characterize induced DNA changes. Conventional methods for insertional deletion identification include (i) enzyme mismatch cleavage (EMC) assays, which do not provide reliable, accurate, and sensitive identification of induced indel's; and (ii) Sanger or next-generation DNA sequencing, which are time-consuming, costly and labor-intensive, and are not suitable for high-throughput screening of hundreds or thousands of clones that typically require selection of desirable multiple allelic editing events that often occur at low frequencies. However, the indel detection by amplicon analysis (IDAA) assay, a method that combines a simple amplicon labeling strategy with the high-throughput capability of DNA fragment analysis by automated capillary electrophoresis, allows for high-throughput, low-cost detection of indels down single base event, estimation of the " cutting" efficiency of targeting tools, and assessment of off-target events at candidate sites.

Fig.1 Schematic depiction of the IDAA strategy. (Yang Z., et al.,2015)

Our Indel Detection by Amplicon Analysis (IDAA) Assay Services

Equipped with experienced scientists and an advanced gene editing validation platform, BioVenic is committed to providing indel detection by amplicon analysis (IDAA) assay services to our customers in an efficient and cost-effective manner. We utilize unparalleled custom protocol design, advanced project lifecycle management, and real-time data to ensure ideal results.

• Validation Process Overview

• Features and Benefits
  
  • Insertion deletion sizes down to ±1 bp can be accurately defined.
  • The IDAA strategy is user-friendly and easily implemented in any standard laboratory.
  • Suitable for detecting indels in both cell pools with low-efficiency targeting and single-sorted cells.
  • Fast and sensitive.

Why Choose Us?

• Our long-standing background in gene editing provides us with significant insight into designing gene editing validation solutions, as well as up-to-date technology trends and innovative global expertise.
• With our comprehensive platform and advanced technology in the field of gene editing validation, we can provide professional and customized solutions to our clients. Our talented team of scientists will support you in every aspect of your project.
• We provide our clients with high-quality indel detection by amplicon analysis (IDAA) assay services to ensure the reliability and validity of your gene editing results.

Want to Learn More?

As an expert in the field of gene editing, BioVenic is committed to providing a full range of gene editing validation services. We desire to leverage our advanced technology and extensive experience to help our customers break the bottleneck of gene editing technology in animal breeding and genetic applications. If you are interested in our services, please contact us for more information and a detailed quote.

Reference

1. Yang Z.; et al. Fast and sensitive detection of indels induced by precise gene targeting. Nucleic Acids Res. 2015, 43(9):e59.