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Mouse Subcutaneous Connective Tissue Cell Line-L Wnt-3A

Product Overview

L Wnt-3A cells are stable transfectants derived from L-M(TK-) cells by transfection with a Wnt-3A expression vector and selected in G418-containing medium. The Wnt-3A gene encodes a secreted glycoprotein with modified signaling efficacy. Wnt genes control numerous pattern formation and growth events during embryonic development. L Wnt-3A cells secrete biologically active Wnt-3A protein and are currently the best source for producing Wnt-3A conditioned medium. Since this conditioned medium also contains factors other than Wnt-3A protein, it's necessary to use the parental cell line as a control for experiments involving Wnt-3A conditioned medium.

Specifications

Cell Product Type Cell Line

Species Mouse

Cell Type Fibroblasts

Source Subcutaneous Connective Tissue

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium DMEM with 10% FBS; 1% Penicillin-Streptomycin Solution; 0.4 mg/mL G418

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.