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Mouse Renal Medullary Cell Line-mlMCD-3

Cat. No.CC7F88

Product TypeAnimal Cells and Cell Lines

Product Overview

The mouse renal medullary cell line mIMCD-3 is an inner medullary collecting duct (IMCD) cell line. The terminal one-third tubules of the IMCD were microdissected and placed in culture. Confluent cells were passaged and cloned using cloning cylinders. This is a polarized epithelial cell line that retains many differentiated characteristics of the terminal IMCD, including inhibition of apical-to-basolateral sodium flux by amiloride and atrial natriuretic peptide (ANP). Through Western blot analysis, it was determined that the cells possess amiloride-sensitive sodium channels and accumulate major organic osmolytes (inositol, sorbitol, betaine, and glycerophosphocholine) in response to hyperosmotic stress. The cells secrete endothelin and form tubules with tight junctions. mIMCD-3 cells are sensitive to hepatocyte growth factor (HGF) and easily adapt to grow in hyperosmotic medium supplemented with NaCl and urea (up to 910 mosmol/kg H2O). These extreme osmotic conditions are present in the renal medulla in vivo but are known to be lethal to most other cells.

Specifications

Cell Product Type Cell Line
Species Mouse
Cell Type Epithelial Cells
Source Kidney
Growth Information Adherent; 37°C; 5%CO2
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Formulation and Cell Culture Medium DMEM/F12; 10% FBS; 1% Penicillin-Streptomycin Solution
Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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