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Mouse Renal Collecting Duct Cell Line-M1

Cat. No.CC7F75

Product TypeAnimal Cells and Cell Lines

Product Overview

Mouse renal collecting duct cell line M1 originated from the normal kidney tissue of tg(SV40E)Bri7 transgenic mice. They contain SV40 viral DNA sequences and retain certain characteristics of cortical collecting ducts, such as morphology and CCD (cortical collecting duct) antigens. Most M-1 cell clones exhibit features of either intercalated cells or principal cells from the cortical collecting duct; 5% to 10% of the cells show a dual phenotype of both intercalated and principal cells. When these cells are grown on a permeable support, they can form lumen-like structures with a negative transepithelial potential difference.

Specifications

Cell Product Type Cell Line
Species Mouse
Cell Type Epithelial Cells
Source Kidney
Growth Information Adherent; 37°C; 5%CO2
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Formulation and Cell Culture Medium D/F12 with 5% FBS; 0.005mM Dexamethasone; 1% Penicillin-Streptomycin Solution
Physical State Frozen cells

Target Information

These cells are transformed and stably express the large T-antigen of the simian virus 40 (SV40T antigens) to immortalize the cells and extend their lifespan.

Target/Biomarker SV40T antigens
Target Synonym large T antigen [ Simian virus 40 ]
Gene ID 1489531

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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