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Mouse Embryonic Fibroblast Cell Line-PA317

Product Overview

Mouse Embryonic Fibroblast Cell Line-PA317 were derived from TK- NIH/3T3 cells. They were constructed through co-transfection with a packaging construct DNA (pPAM3) in pBR322 and the herpes simplex virus thymidine kinase gene, also in pBR322. Introduction of retroviral vectors into these cells, either by infection or transfection, results in the production of amphotropic vector particles capable of infecting many mammalian cell types.

Specifications

Cell Product Type Cell Line

Species Mouse

Cell Type Fibroblasts

Source Embryo

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium DMEM with 10% FBS; 1% Penicillin-Streptomycin Solution

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.