Products

Get a quote

We're excited to learn more about your project and provide you with a customized quote tailored to your needs. Please fill out the form below, and we'll get back to you as soon as possible.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Mouse Embryonic Stem Cells-E14TG2a

Product Overview

Mouse Embryonic Stem Cells-E14TG2a are embryonic stem cells derived from the inner cell mass of a 129/Ola strain mouse embryo. These cells lack HGPRT (HPRT) and are resistant to 0.06 mM 6-thioguanine. When cultured on a feeder layer (embryonic fibroblasts or STO cells), the cells remain undifferentiated. In the absence of a feeder layer, the cells spontaneously differentiate and form embryoid bodies. When injected into blastocysts, the cells can contribute to the germline. Following conventional molecular gene modification techniques, they can be used to reconstitute mouse embryos.

Specifications

Cell Product Type Embryonic Stem Cells

Species Mouse

Cell Type Stem Cells

Source Embryo

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium DMEM with 15% FBS; 0.1 μL/mL LIF; 1% L-Glutamine; 1% NEAA; 1% Sodium pyruvate; 1% PS; 1 μL/mL β-Mercaptoethanol; 1% Penicillin-Streptomycin Solution. The culture flask needs to be coated with 0.1% gelatin. The cells grow in colonies.

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.