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Rat Alveolar Type II Epithelial Cell Line-RLE-6TN

Product Overview

Rat Alveolar Type II Epithelial Cell Line-RLE-6TN was isolated from 56-day-old male F344 rats by airway perfusion with a streptolysin solution. Expression of the SV40-T antigen was negative by nuclear immunostaining and PCR, indicating that these cells were obtained through spontaneous immortalization. This cell line exhibits characteristics of alveolar type II cells, such as lipid inclusions (identified by Phosphine 3R staining and electron microscopy) and the expression of cytokeratins 8 and 19. The cells do not express alkaline phosphatase activity. Several chemokines expressed by RLE-6TN cells have been reported to be similar to those in primary cultures of alveolar type II cells. They are not tumorigenic in nude mice.

Specifications

Cell Product Type Cell Line

Species Rat

Cell Type Epithelial Cells

Source Lung

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium F12 with 10% FBS; 1% ITS; 5 ng/mL EGF; 1% Penicillin-Streptomycin Solution

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.