Mouse Colon Carcinoma Cell Line-CT26.CL25

CT26 cells are an undifferentiated type of colon cancer cell line derived from a BALB/c mouse, following induction by N-nitroso-N-methylurethane (NNMU). Known for their high tumorigenic potential, these cells are capable of forming tumors in syngeneic mice, thus serving as an important model for researching tumor growth, metastasis, and immune responses. CT26.WT was stably transformed by the retroviral vector LXSN, which contains the lacZ gene, encoding a tumor-associated antigen (TAA) and beta-galactosidase. This transformation resulted in the creation of a lethal subcloned cell line, CT26.CL25. While CT26.WT and CT26.CL25 cells exhibit similar growth rates and lethality in mice, CT26.CL25 uniquely expresses the tumor-associated antigen and beta-galactosidase.