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Rat Myoblast Cell Line-L6

Product Overview

Rat Myoblast Cell Line-L6 was originally isolated from the first two generations of primary cultures of rat thigh muscle, grown in the presence of methylcholanthrene. In culture, L6 cells fuse to form multinucleated myotubes and striated muscle fibers. The degree of cell fusion decreases with increasing passage number, so these cells should be frozen at low passages and periodically re-cloned to select for cells with strong fusion capabilities. If the cells are allowed to overgrow in the culture vessel, the myogenic component of this cell line will rapidly deplete. Testing has shown these cells are negative for ectromelia virus (mousepox).

Specifications

Cell Product Type Cell Line

Species Rat

Cell Type Myoblasts

Source Thigh Muscles

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium DMEM with 10% FBS; 1% Penicillin-Streptomycin Solution

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.