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Mouse Mononuclear Macrophage Leukemia Cell Line-RAW 264.7-GFP

Cat. No.CC7F113

Product TypeAnimal Cells and Cell Lines

Product Overview

The RAW 264.7 mouse monocyte/macrophage leukemia cell line originated from a tumor induced by the Abelson murine leukemia virus. These cells are negative for sIg and Ia antigens and the Thy-1.2 surface antigen. This cell line does not secrete detectable viral particles and is negative in the XC plaque-forming assay. RAW 264.7 cells can pinocytose neutral red and phagocytose latex beads and zymosan. They are capable of antibody-dependent degradation of sheep red blood cells and tumor target cells. Treatment with LPS or PPD for two days can induce the degradation of red blood cells, but does not affect tumor target cells. These cell products stably expressed GFP which transfected with lentivirus, producing a vivid green fluorescence when exposed to blue light. This fluorescence can be utilized for visualization purposes in various related research applications.

Specifications

Cell Product Type Cell Line
Species Mouse
Cell Type Tumor Cell Line
Source Monocyte Macrophage Leukemia
Disease Monocyte Macrophage Leukemia
Growth Information Adherent; 37°C; 5%CO2
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Formulation and Cell Culture Medium DMEM with 10% FBS; 1% Penicillin-Streptomycin Solution. Cannot be digested with trypsin, easy to differentiate, high density is required for passaging.
Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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