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Mouse Hepatic Stellate Cells-Immortalized by SV40T Antigens
Product Overview
Hepatic stellate cells (HSCs) are located within the space of Disse, close to hepatic sinusoidal endothelial cells and hepatocytes. They have an irregular morphology, with round or irregularly shaped cell bodies often extending several star-like processes that surround the hepatic sinusoids. Additionally, HSCs extend processes to contact hepatocytes and neighboring stellate cells. When the liver is damaged by inflammation or mechanical stimulation, hepatic stellate cells become activated. Their phenotype changes from a quiescent to an activated state. Activated hepatic stellate cells, on one hand, contribute to the formation of liver fibrosis and the remodeling of intrahepatic structures through proliferation and extracellular matrix secretion. On the other hand, they increase intrasinusoidal pressure via cell contraction. Mouse hepatic stellate cells were immortalized through lentiviral transfection carrying the SV40 gene (these cells acquire puromycin resistance after transfection).
Specifications
Target Information
These cells are transformed and stably express the large T-antigen of the simian virus 40 (SV40T antigens) to immortalize the cells and extend their lifespan.
Shipping and Storage
This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.