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Chicken Lymphoma Cell Line-DT40

Product Overview

DT40 cells are a chicken bursal lymphocyte cell line established through induction by avian leukosis virus from Hyline SC chickens. The original lymphoma was obtained by infecting one-day-old chicks with Rous-associated virus 1 (RAV-1). Tumors formed in the bursa of Fabricius were prepared as a cell suspension and then intravenously injected into syngeneic recipient chicks. After a single in vivo transplantation, the DT40 cell line was established. This cell line exhibits a lymphoblastoid phenotype. Infectivity tests indicate that DT40 releases low levels of infectious RAV-1. This cell line can be used for stable transfection studies.

Specifications

Cell Product Type Cell Line

Species Chicken

Cell Type Lymphoblasts

Source Lymphoma

Disease Lymphoma

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium DMEM (containing NaHCO3 1.5 g/L) with 10% FBS; 5% Chicken serum; 0.05 mM β-Mercaptoethanol; 4 mM L-Glutamine; 1% Penicillin-Streptomycin Solution

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.