Zebrafish Staining Products

Introduction

Antibodies

High-quality antibodies are the bedrock of reliable immunofluorescence and immunohistochemistry experiments in zebrafish, enabling precise visualization of specific proteins. BioVenic specializes in the discovery, engineering, and manufacturing of veterinary and species-specific antibodies tailored for research applications, including those optimized for fish models. Our services cover the full workflow, from antigen preparation to antibody production via platforms like hybridoma and single B cell technology. This dedication ensures the creation of antibodies with exceptional specificity and low background noise, which is crucial for whole-mount staining of delicate zebrafish embryos and tissues. Researchers can rely on BioVenic for custom primary and secondary antibodies essential for mapping protein expression patterns during development or disease progression.

Staining Kits

To streamline laboratory workflows and ensure consistency, BioVenic offers various detection and assay kits designed to assess key biological processes relevant to zebrafish studies. While we provide general animal ELISA kits for quantifying proteins, our capabilities also extend to developing specialized staining kits. These kits often focus on critical indicators such as cell viability, apoptosis, and proliferation, which are essential for drug toxicity screening in zebrafish larvae. Furthermore, our expertise in developing Fluorescent In Situ Hybridization (FISH) assays provides specialized kits and services for detecting target nucleic acid sequences, allowing for precise localization of specific DNA or RNA within zebrafish cells and tissues.

Genetic Manipulation

Genetic manipulation is fundamental to modern zebrafish research, creating transgenic lines or targeted mutants that often require staining techniques to validate expression or phenotype. BioVenic provides sophisticated services for animal genetic modification and genome editing, including knockout, knock-in, and point mutation creation in animal stem cells and models. By providing high-quality genetically modified cells and validation services, we supply the research community with the complex models needed for expression studies. These services are invaluable for developing reporter lines, where the visualization of fluorescent proteins through staining techniques is the primary readout of gene regulatory mechanisms or disease processes.

Nucleic Acid Extraction & Purification

Accurate and reproducible staining protocols often necessitate high-quality nucleic acids for upstream or downstream molecular applications. BioVenic offers comprehensive services and kits for animal nucleic acid extraction and purification, including high-efficiency genomic DNA and total RNA purification from diverse animal samples, including fish tissues and cells. We employ advanced technology platforms such as spin column and magnetic bead-based methods, ensuring high purity, yield, and structural integrity of the extracted nucleic acids. This critical foundation supports sensitive applications like quantitative PCR (qPCR), transcriptome sequencing, and the preparation of high-quality probes for in situ hybridization (ISH) assays.

Other Detection & Labeling

Beyond standard immunodetection, BioVenic offers advanced omics solutions that include specialized detection and labeling services. Our highly sensitive Animal Metabolomics Solutions, for instance, utilize techniques like LC-MS to qualitatively and quantitatively profile metabolites, which can be linked to staining readouts of cellular health and metabolism. We also provide customized cell-based assays, such as cytotoxicity and apoptosis assays, which frequently rely on fluorescent dyes and labeling reagents to assess cell fate and toxicity effects in live zebrafish cell lines or primary cell cultures, providing essential data for veterinary drug development and pathology studies.

Advantages

BioVenic ensures client success in zebrafish research through distinct advantages in product quality and service:

• Customized, One-Stop Solution Platform: We offer fully integrated services, handling projects from initial design (e.g., custom antigen preparation) to final product delivery (e.g., custom antibody or kit manufacturing), streamlining complex research for our clients.
• Stringent Quality Control and Expert Team: Our processes are governed by strict Standard Operating Procedures (SOPs), utilizing powerful detection technologies and an expert team to ensure every product, whether an antibody or a nucleic acid kit, meets the highest benchmarks for reliability, purity, and activity.
• Breadth of Multi-Omics and Technical Expertise: We integrate expertise across genomics, transcriptomics, proteomics, and metabolomics to provide comprehensive solutions. This integrated approach allows us to better support complex staining studies that correlate visual data with underlying molecular mechanisms.

Our products and services are for research use only and cannot be used for any clinical purposes.

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