- Email:
- Phone:
- Add:
Aquatic Animal Gene Knockdown Service
The basic principle of gene knockout technology is similar to gene knockout, but the goal is to reduce the expression level of the target gene rather than completely eliminate it. BioVenic has extensive experience in gene editing of aquatic animals and provides standardized gene knockdown services to meet your needs in various scientific scenarios.
Gene knockdown is a common research method in the scientific study of aquatic animals, typically achieved through RNAi, siRNAs and modified antisense oligonucleotides. Knockdown methods target the mRNA rather than alter the DNA.
Fig. 1
Overview of knockdown and loss-of-function technologies in zebrafish. (Pauli A.; et al., 2015)
In RNAi, the introduction of particular siRNA or shRNA molecules selectively silences the transcription of target gene mRNA, thereby reducing the expression levels of the target gene. Anti sense oligonucleotides(ASO) are synthetic single stranded DNA or RNA fragments that can complement and bind to specific sequences of target mRNA. Through this binding, antisense oligonucleotides prevent mRNA translation or promote its degradation, thereby reducing the expression of the target gene. Morpholino oligonucleotides (MOs) are widely used for targeted gene knockdowns in zebrafish due to their simplicity and effectiveness. MOs are DNA analogues with a modified backbone, replacing ribose with morpholine, which enhances stability and prevents degradation without affecting RNA binding.
| Methods | Scope of Application |
| RNA interference (RNAi) | Suitable for a wide range of gene function research, the technology is mature but delivery and stability issues need to be optimized. |
| Anti sense oligonucleotides (ASO) | Strong flexibility, suitable for precise regulation of gene expression, but high cost and potentially unstable effectiveness. |
| Morpholino oligonucleotides (MOs) | Good stability, suitable for early gene function research, but delivery and cost are the main considerations. |
By utilizing the latest gene knockdown technology, we are able to precisely control the expression of target genes and provide excellent gene knockdown services for aquatic animals.
Professional Team
Customized Services
Innovative Technology
Efficient Services
BioVenic is committed to supporting our clients' scientific research projects through efficient and precise gene editing technology, ensuring the provision of customized services that meet their needs and expectations. No matter what your challenge is, we are confident that we can provide you with the best solution. If you are seeking a reliable partner to support your aquatic animal research projects, please do not hesitate to contact us.
Reference
Our products and services are for research use only and cannot be used for any clinical purposes.
