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Aquatic Animal Hepatotoxicity Assay Service
The liver is a major organ for drug metabolism, and many drugs or chemicals may cause damage to liver cells. Consequently, hepatotoxicity assessment is crucial in drug development and safety studies. BioVenic is dedicated to providing personalized research solutions and technical support to meet the diverse needs of research teams.
Aquatic animal hepatotoxicity assay is an experimental in vitro or in vivo method for assessing the toxic effects of compounds, drugs or other exposure factors on liver cells. The hepatotoxicity assay boasts many features and benefits that set it apart from other assays on the market. Chief among these is its high throughput screening capacity, which allows for rapid and efficient analysis. The assay combines a variety of multi-parameter analyses, including biochemical analyses, cell viability assays, and other methods, to provide a comprehensive toxicity assessment.
Fig. 1 Assess the hepatotoxicity of phosphogypsum leachate in zebrafish. (Wang, et al., 2024)
Using aquatic animal as research subjects, such as tilapia and rainbow trout, hepatotoxicity assay plays a key role in biotransformation and detoxification processes, and assessing hepatotoxicity critical to understanding environmental pollution and ecological health. Applications of this test include but are not limited to, the use of aquatic organism models to study the mechanisms of human liver disease, the development of relevant new drugs, and the assessment of aquaculture feed safety.
Aquaculture and Fishing
Disease Research Model
Drug Development
Environment and Ecology
The hepatotoxicity assay provides a comprehensive range of assays and analyses to assess the effects of environmental pollutants, drugs, feed, and other factors on the liver of aquatic organisms. BioVenic offers flexible and professional experimental design and data analysis services to meet your specific research requirements.
Cell lines or primary hepatocytes are frequently used in in vitro experiments to assess the toxic effects of drugs, chemicals, or environmental pollutants on the liver.
Biochemical Assay Technologies
Liver enzymes such as ALT and AST, which react to liver cell damage, are tested, along with metabolites such as bilirubin, bile acids, and glucose. These are used to determine abnormalities in liver metabolism.
Cytotoxicity and Apoptosis Assay
The viability, apoptosis, and necrosis ratios of hepatocytes were assessed by combining MTT/CCK-8, Annexin V/PI double staining, flow cytometry, and LDH release assay to reflect hepatocyte membrane damage, thus studying the mechanism of toxicity.
Experiments in vivo can provide insights into how pollutants and drugs affect aquatic organisms' livers and their physiological responses.
Intoxication Kinetics
Metabolic pathways, changes in liver concentration and biological half-life of the substance in aquatic organisms will be monitored after the chemical has been labelled and indicators such as serum concentration and changes in liver enzyme induction will be assessed.
Behavior Assessment
Indicators of behavioral changes are assessed by observing the physiological responses of aquatic organisms in the polluted environment, such as swimming behavior, mobility, foraging behavior, etc., and indirectly by determining liver damage and general health.
Professional Team
Customized Services
Innovative Technology
Efficient Services
BioVenic employs advanced assay technology to ensure the accuracy of all data, which enhances the scientific rigor and reliability of your research efforts. We conduct comprehensive technical to gain insight into the effects of pollutants and drugs on the liver of aquatic organisms, as well as their physiological responses. Please feel free to contact us if you need.
Reference
Our products and services are for research use only and cannot be used for any clinical purposes.