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Mouse Subcutaneous Connective Tissue Cell Line-L WRN

Cat. No.CC7F74

Product TypeAnimal Cells and Cell Lines

Product Overview

L-WRN is a fibroblast-like cell line isolated from the mammary fat pad of a male mouse. This cell line is a source for producing conditioned medium containing Wnt-3A, R-spondin 3, and Noggin, which can be used to culture various mammalian tissue stem cells. L-WRN cells were obtained by co-transfecting L-Wnt3A cells with R-spondin 3 and Noggin co-expression vectors and selecting stable clones in medium containing G418 and hygromycin B. L-WRN cells secrete the factors Wnt3A, R-spondin 3, and Noggin into the culture medium. Wnt3A binds to the frizzled receptor family and activates beta-catenin-dependent transcription. Members of the R-spondin protein family are potent co-activators of canonical Wnt signaling in the intestine and are essential for isolating small intestinal stem cells. Noggin is a bone morphogenetic protein (BMP) signaling inhibitor that can maintain and propagate small intestinal organoids in vitro.

Specifications

Cell Product Type Cell Line
Species Mouse
Cell Type Fibroblasts
Source Subcutaneous Connective Tissue
Growth Information Adherent; 37°C; 5%CO2
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Formulation and Cell Culture Medium DMEM with 10% FBS; 1% Penicillin-Streptomycin Solution; 0.5 mg/mL G418; 0.5 mg/mL hygromycin B
Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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