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Mouse Mononuclear Macrophage Leukemia Cells-RAW 264.7-Luc-GFP-Puro

Cat. No.CC8F45

Product TypeAnimal Cells and Cell Lines

Product Overview

The Raw264.7 cell line was established from tumor tissue induced by Abelson murine leukemia virus, and it's one of the most commonly used inflammatory cell models in research. These cells are easy to proliferate, have high DNA transfection efficiency, and are sensitive to RNA interference. They are frequently used as host cells for transfection and for replicating murine norovirus. The cell surface is negative for sIg-, Ia-, and Thy-1.2 antigens. They can antibody-dependently lyse sheep red blood cells and tumor target cells. After two days of treatment with LPS or PPD, they can be induced to lyse red blood cells, but they have no effect on tumor target cells. Raw264.7-LUC-GFP-Puro cells are a stable cell line derived from Raw264.7 cells infected with lentivirus, resulting in the stable expression of firefly luciferase and GFP.

Specifications

Cell Product Type Cell Line
Species Mouse
Cell Type Macrophages
Source Ascites
Disease Leukemia Virus-induced Tumor
Growth Information Adherent; 37°C; 5%CO2
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Formulation and Cell Culture Medium DMEM high glucose (containing sodium pyruvate), 10% FBS
Physical State Frozen cells

Target Information

This cell line is a cell line stably expressing firefly luciferase and GFP obtained by infecting Raw264.7 cells with lentivirus.

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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