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Mouse Embryonic Fibroblast Cell Line-NIH/3T3-Luc-GFP-Puro

Product Overview

Mouse Embryonic Fibroblast Cell Line-NIH/3T3 is a highly contact-inhibited, continuous cell line established from NIH Swiss mouse embryo cultures. To cultivate substrains morphologically more suitable for transformation analysis, the established NIH/3T3 cell line underwent more than five rounds of subcloning. This cell line is handy for DNA transformation and transfection studies. The cells are NIH/3T3 cells infected with lentivirus and stably express firefly luciferase and GFP.

Specifications

Cell Product Type Cell Line

Species Mouse

Cell Type Fibroblasts

Source Embryo

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium DMEM high glucose (with sodium pyruvate), 10% neonatal bovine serum

Physical State Frozen cells

Target Information

This cell line is a cell line stably expressing firefly luciferase and GFP obtained by infecting NIH/3T3 cells with lentivirus.

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.