Products

Get a quote

We're excited to learn more about your project and provide you with a customized quote tailored to your needs. Please fill out the form below, and we'll get back to you as soon as possible.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Mouse Embryonic Fibroblast Cell Line-MEF (Treated by Mitomycin C)

Product Overview

The Mouse Embryonic Fibroblast Cell Line-MEF is established by culturing embryos from 9-day pregnant 615 mice after removing organs such as the brain and heart. These cells can be used as feeder cells to support the growth of Embryonic Stem (ES) cells and maintain their undifferentiated state. When used as feeder cells, MEFs need to be treated with mitomycin C to stop their proliferation. It is recommended that MEFs, when serving as feeders for ES cells, should not exceed six passages. MEF requires mitomycin C treatment to stop growth.

Specifications

Cell Product Type Cell Line

Species Mouse

Cell Type Fibroblasts

Source Embryo

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium D/F12 with 10% FBS; 1% Penicillin-Streptomycin Solution

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.