Mouse Cardiomyocyte Cell Line-HL-1

Mouse Cardiomyocyte Cell Line-HL-1 was established in 1997 from a subcutaneous tumor excised from an adult female C57BL/6J inbred mouse. This mouse was transgenic, having been transfected with a fusion gene consisting of the atrial natriuretic factor promoter and the SV40 region. HL-1 cells are an immortalized mouse cardiomyocyte cell line capable of continuous division and spontaneous contraction while maintaining a differentiated cardiac phenotype. HL-1 cells can be serially passaged without losing their differentiated, specific cardiomyocyte phenotype, including morphological, biochemical, and electrophysiological characteristics. They are commonly used to study the functions of normal cardiomyocytes in signal transduction, cell metabolism, and transcriptional regulation, as well as to simulate pathological conditions in vitro such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.