Genetic Preservation
Genes are the carrier of all biological information of animals, and pet cloning is a long-term and complex experimental process that requires us to keep pet genes intact for a long time. In other words, gene preservation is an important step in the process of pet cloning. It is necessary for us to immediately store our pets' genetic material DNA for long-term use after the pet's death.
The elements of our environment, such as oxygen, sunlight and temperature, will decompose DNA. In addition, various microorganisms in nature are also the "natural enemies" of DNA. Therefore, it is necessary to isolate these elements in order to preserve genes for a long time and maintain their stability. In general, the long-term preservation of genes mainly includes the following techiques:
Liquid Nitrogen Preservation
Liquid nitrogen preservationcanpreserve gene samples at low temperatures in liquid nitrogen below -180℃. Its advantage is that it can realize long-term preservation and meet the needs of gene detection and gene therapy in the future. In other words, once stored in liquid nitrogen, the sample will remain active and can be stored indefinitely.
Electric Refrigeration Preservation
Electric refrigeration storage is usually stored ina-80℃ environment, which requires professional storage equipment, such asa-80℃ refrigerator. Scientific research institutions often use this preservation method to preserve biological samples for a long time, but the preservation period is slightly shorter than that of liquid nitrogen preservation.
Low Humidity Dormancy Preservation
The low humidity dormancy preservation can seal the purified DNA in the DNA capsule device and store it in the refrigerator. The price of this technology is low, and the preserved DNA can also meet the use demand. However, it is easily affected by the storage conditions of the refrigerator. Once the refrigerator is powered off, the quality of the preserved products is affected.
Nano Molecular Coating
Nano molecular coating technique, by collecting oral exfoliated oral mucosal epithelial cells, extracts DNA molecular particles, and wraps a coating layer on the surface to form a core-shell structure, so that the prepared gene preservation products have good stability and can be preserved at room temperature. Gene preservation products made by this method can meet the needs of accurate comparative detection of diseases in the future.
Preservation of Dried Blood Tablets
This method is relatively simple to sample and has low storage difficulty. It can be stored at room temperature. The disadvantage is that the preservation time is short. The DNA preserved will degrade under the action of air and microorganisms without extraction and purification.
Solid-Phase Adsorption
Solid-phase adsorption is to lyse the cells in the sample and extract DNA. After repeated washing and precipitation, impurities such as protein and RNA affecting DNA purity are removed to obtain high-purity DNA.Solid gene products are sealed and stored in ampoules filled with nitrogen. After light avoidance treatment, moisture-proof protection and seismic design, the gene products can be stored stably at room temperature for a long time.
BioVenic provides dogs' and cats' gene preservation to ensure that your pet's genetic material or DNA is preserved in a viable and usable form. This is usually achieved by freezing tissues, individual cells or germs in liquid nitrogen. The genetic material is sealed in a specially designed tube so that tissue culture can be carried out when it reaches its destination. BioVenic allows species protection and ensures that precious animal genes are never lost, as well as DNA for verification once cloned offspring are born. And we strive to contribute to the rapid breeding and genetic preservation of excellent pet animals.
References
- Oh HJ, et al. The promise of dog cloning. Reprod Fertil Dev. 2017 Jan;30(1):1-7.
- Lee SH, et al. Dog cloning-no longer science fiction. Reprod Domest Anim. 2018 Nov;53 Suppl 3:133-138.
- Ayala-Zermeño MA,et al. Viability, purity, and genetic stability of entomopathogenic fungi species using different preservation methods. Fungal Biol. 2017 Nov;121(11):920-928.
- Ivanova EA, et al. The preservation of microbial DNA in archived soils of various genetic types. PLoS One. 2017 Mar 24;12(3):e0173901.