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Recombinant Swine Alpha-Fetoprotein (AFP), N-His-GST

Cat. No.AP9C915

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Swine Alpha-Fetoprotein (AFP), N-His-GST is a recombinant protein expressed from E.coli. Its predicted molecular weight is 52.9 kDa. The purity is>95% (SDS-PAGE).

Specifications

Type Recombinant Protein
Species Swine
Expression System E.coli
Purity >95% (SDS-PAGE)
Endotoxin < 1 EU/µg
Predicted Molecular Weight 52.9 kDa
Molecular Weight 50.0 kDa
Physical State Lyophilized
Formulation The buffer before lyophilization is PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Target Information

Swine alpha-fetoprotein (AFP) is a fetal serum glycoprotein primarily synthesized in the fetal liver and yolk sac, known for its high levels during pregnancy. It plays a significant role in the development of hepatocellular carcinoma (HCC), promoting cell proliferation, invasion, and metastasis while inhibiting apoptosis. AFP also has immunosuppressive functions, influencing immune cell activities and promoting immune escape of cancer cells.

Protein Swine Alpha-Fetoprotein (AFP)
Protein Synonym aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
Gene ID 397586
UniProt ID Q8MJ76

Shipping and Storage

This product is shipped with dry ice. It is recommended to aliquote as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL.Aliquote the reconstituted solution to minimise freeze-thaw cycles.

References

  1. He, Z, and M Feng. et al. Activation, isolation, identification and culture of hepatic stem cells from porcine liver tissues. Cell proliferation. 2011, 44,6: 558-66.
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