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Recombinant Porcine Creatine Kinase, Muscle (CKM), N-His-GST

Cat. No.AP7F187

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Porcine Creatine Kinase, Muscle (CKM), N-His-GST is a recombinant protein expressed from E.coli expression system. Its predicted molecular weight is 70.3 kDa. The purity is>90% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL). It can be used as a positive control or as an immunogen.

Specifications

Type Recombinant Protein
Species Swine
Expression System E.coli
Purity >90% (SDS-PAGE)
Endotoxin <1 EU/μg (LAL)
Predicted Molecular Weight 70.3 kDa
Molecular Weight 70 kDa
Physical State Lyophilized
Formulation PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Target Information

Creatine kinase M-type (CK-MM), also known as muscle-type creatine kinase, is an enzyme expressed in various tissues and cell types. Creatine Kinase M-Type is encoded by the CKM gene in pigs. The CKM gene is driven by its promoter only at the final stage of muscle differentiation, so its expression is temporally and spatially specific. Research has shown that the CKM gene may serve as a potential muscle-specific safe harbor locus in the porcine genome.

Protein Porcine Creatine Kinase, Muscle (CKM)
Protein Synonym CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed
Gene ID 397264
UniProt ID Q5XLD3
EC No. 2.7.3.2

Shipping and Storage

This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 4 weeks at 2-8°C, for 12 months at -80°C.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Aliquote the reconstituted solution to minimise freeze-thaw cycles.

References

  1. Xiong, Y. et al. Identification of the CKM Gene as a Potential Muscle-Specific Safe Harbor Locus in Pig Genome. Genes. 2022, 13.
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